Erban Tomas, Ledvinka Ondrej, Nesvorna Marta, Hubert Jan
Crop Research Institute, Prague, Czechia.
Czech Hydrometeorological Institute, Prague, Czechia.
Appl Environ Microbiol. 2017 Apr 17;83(9). doi: 10.1128/AEM.00128-17. Print 2017 May 1.
is inhabited by bacteria that differ among mite populations (strains) and diets. Here, we investigated how the microbiome and fitness of are altered by dietary perturbations and mite populations. Four populations, referred to as dog, Koppert, laboratory, and Phillips, underwent a perturbation, i.e., a dietary switch from a rearing diet to two experimental diets. The microbiome was investigated by sequencing the V1-V3 portion of the 16S rRNA gene, and selected bacterial taxa were quantified by quantitative PCR (qPCR) using group/taxon-specific primers. The parameters observed were the changes in mite population growth and nutritional status, i.e., the total glycogen, lipid, saccharide, and protein contents in mites. The effect of diet perturbation on the variability of the microbiome composition and population growth was lower than the effect induced by mite population. In contrast, the diet perturbation showed a greater effect on nutritional status of mites than the mite population. The endosymbionts exhibited high variations among populations, including in the laboratory population, -like bacteria in the dog population, and in the dog and Phillips populations. -like and -like bacteria were present in the dog, Koppert, and Phillips populations in different proportions. The microbiome is dynamic and varies based on both the mite population and perturbation; however, the mites remain characterized by robust bacterial communities. Bacterial endosymbionts were found in all populations but represented a dominant portion of the microbiome in only some populations. We addressed the question of whether population origin or perturbation exerts a more significant influence on the bacterial community of the stored product mite The microbiomes of four populations of insects subjected to diet perturbation were compared. Based on our results, the bacterial community was more affected by the mite population than by diet perturbation. This result can be interpreted as indicating high stability of the putative intracellular symbionts in response to dietary perturbation. The changes in the absolute and relative numbers of , -like, -like, and bacteria in the populations can also be caused by neutral processes other than perturbation. When nutritional status is considered, the effect of population appeared less important than the perturbation. We hypothesize that differences in the proportions of the endosymbiotic bacteria result in changes in mite population growth.
螨虫种群（品系）和食物不同，其体内栖息的细菌也不同。在此，我们研究了饮食扰动和螨虫种群如何改变螨虫的微生物组和健康状况。四个螨虫种群，即犬类、科伯特、实验室和菲利普斯种群，经历了一次扰动，即从饲养食物转换为两种实验性食物。通过对16S rRNA基因的V1 - V3部分进行测序来研究微生物组，并使用组/分类群特异性引物通过定量PCR（qPCR）对选定的细菌分类群进行定量。观察的参数是螨虫种群增长和营养状况的变化，即螨虫体内总糖原、脂质、糖类和蛋白质含量。饮食扰动对微生物组组成变异性和种群增长的影响低于螨虫种群所诱导的影响。相比之下，饮食扰动对螨虫营养状况的影响大于螨虫种群。内共生菌在螨虫种群中表现出高度变异，包括实验室种群中的某种菌、犬类种群中的类某菌，以及犬类和菲利普斯种群中的另一种菌。类某菌和类某菌以不同比例存在于犬类、科伯特和菲利普斯种群中。螨虫微生物组是动态的，会因螨虫种群和扰动而变化；然而，螨虫仍以强大的细菌群落为特征。在所有种群中都发现了细菌内共生菌，但仅在某些种群中它们占微生物组的主导部分。我们探讨了种群起源或扰动对储粮螨虫细菌群落影响更大这一问题。比较了经历饮食扰动的四个螨虫种群的昆虫的微生物组。根据我们的结果，细菌群落受螨虫种群的影响大于饮食扰动。这一结果可解释为表明假定的细胞内共生菌对饮食扰动具有高稳定性。螨虫种群中某种菌、类某菌、类某菌和另一种菌的绝对和相对数量变化也可能由扰动以外的中性过程引起。当考虑营养状况时，种群的影响似乎不如扰动重要。我们假设内共生菌比例的差异会导致螨虫种群增长的变化。
