Dept. of Bio and Nano Technology, Guru Jambheshwar University of Science and Technology, Hisar, Haryana 125001, India.
Dept. of Bio and Nano Technology, Guru Jambheshwar University of Science and Technology, Hisar, Haryana 125001, India.
Sci Total Environ. 2017 May 15;586:1272-1278. doi: 10.1016/j.scitotenv.2017.02.138. Epub 2017 Feb 22.
The nanoformulation of pesticides has the potential to increase food productivity, while resolving the drawbacks of conventional agrochemicals, which have negative environmental impacts. In this study, herbicide (metsulfuron methyl)-loaded pectin (polysaccharide) nanoparticles were synthesized and evaluated for herbicidal activity and cytotoxicity. The optimum formulation of nanoparticles was obtained using the Central Composite Design. The basic properties (mean particle size, stability, morphology, and interaction between polymer and herbicide) were characterized using a particle size analyzer (PSA), zeta potential, transmission electron microscopy (TEM), and Fourier Transform infrared spectroscopy (FTIR), respectively. The nanoparticles were found to be in size range of 50-90nm with zeta potential value of -35.9mV. The herbicide loading and herbicide encapsulation efficiency of the nanoparticles were determined to be 6.30% and 63±2%, respectively. The cytotoxicity of the herbicide-loaded nanoparticles was evaluated using healthy cell lines (Vero cell lines) and compared with that of commercial herbicide. In addition, an in-field evaluation of our nanoformulation's effects on the Chenopodium album plant was performed using a pectin nanocarrier. The results showed that application of herbicide-loaded nanoparticles could be used to reduce the use of herbicides with improved efficacy and environmental safety.
农药的纳米制剂有可能提高粮食产量，同时解决传统农药对环境的负面影响。在这项研究中，合成了负载除草剂（甲磺隆甲酯）的果胶（多糖）纳米粒子，并对其除草活性和细胞毒性进行了评价。使用中心复合设计得到了纳米粒子的最佳配方。使用颗粒大小分析仪（PSA）、Zeta 电位、透射电子显微镜（TEM）和傅里叶变换红外光谱（FTIR）分别对纳米粒子的基本性质（平均粒径、稳定性、形态和聚合物与除草剂之间的相互作用）进行了表征。纳米粒子的粒径在 50-90nm 之间，Zeta 电位值为-35.9mV。纳米粒子的载药量和除草剂包封效率分别为 6.30%和 63±2%。使用健康细胞系（Vero 细胞系）评价了负载除草剂的纳米粒子的细胞毒性，并与商业除草剂进行了比较。此外，还使用果胶纳米载体对我们的纳米制剂对藜草植物的田间效果进行了评价。结果表明，负载除草剂的纳米粒子的应用可以减少除草剂的使用，提高药效和环境安全性。
