Zhang Liang, Guo Gangqiang, Xu Jianfeng, Sun Xiangwei, Chen Wenjing, Jin Jinji, Hu Changyuan, Zhang Peichen, Shen Xian, Xue Xiangyang
Department of Gastrointestinal Surgery, the First Affiliated Hospital of Wenzhou Medical University, Ouhai District, 325000, Wenzhou, Zhe Jiang, P.R.China.
Department of Microbiology and Immunology, Institute of Molecular Virology and Immunology, Institute of tropical medicine, Wenzhou Medical University, Ouhai District, 325000, Wenzhou, Zhe Jiang, P.R.China.
Diagn Microbiol Infect Dis. 2017 May;88(1):62-68. doi: 10.1016/j.diagmicrobio.2017.02.001. Epub 2017 Feb 8.
Increasing evidence suggests that human cytomegalovirus (HCMV) is associated with many human malignancies. However, its prevalence in gastric cancer (GC) and clinical association remain unknown. HCMV IgG and IgM antibodies in the sera of 80 GC patients and 80 healthy controls were detected using a microparticle enzyme immunoassay. The prevalence of HCMV UL47, UL55, UL56, and UL77 genes among 102 GC tumor tissues and adjacent normal specimens was measured by polymerase chain reaction (PCR) or nested PCR. Quantitative real-time PCR (Q-PCR) was used to determine viral load. Virus localization in neoplastic tissues was determined by immunohistochemistry. No significant difference of HCMV IgG and IgM seropositivity was found between GC patients and the healthy group. However, the overall HCMV DNA positivity rate was significantly higher in GC cancerous tissue compared with in paired normal tissue (P<0.01). HCMV infection was mainly localized in the tumorous epithelium. Q-PCR in HCMV-positive specimens indicated that the viral copy number was notably higher in GC tissues than in adjacent normal specimens (P<0.001). Clinical statistical analysis indicated that HCMV load in GC tumor tissue was positively associated with lymphatic metastasis (P=0.043), the area under the receiver operating characteristic (ROC) curve was 0.6638. Our data clearly provide the prevalence of HCMV in GC patients. We conclude that HCMV infection in malignant tissues might be associated with carcinogenesis or progression of GC and possibly relates to lymphatic metastasis.
越来越多的证据表明，人巨细胞病毒（HCMV）与许多人类恶性肿瘤相关。然而，其在胃癌（GC）中的流行情况及临床关联仍不清楚。采用微粒酶免疫分析法检测了80例GC患者和80例健康对照者血清中的HCMV IgG和IgM抗体。通过聚合酶链反应（PCR）或巢式PCR检测了102例GC肿瘤组织及相邻正常标本中HCMV UL47、UL55、UL56和UL77基因的流行情况。采用定量实时PCR（Q-PCR）测定病毒载量。通过免疫组织化学确定肿瘤组织中的病毒定位。GC患者与健康组之间HCMV IgG和IgM血清阳性率无显著差异。然而，与配对的正常组织相比，GC癌组织中HCMV DNA总体阳性率显著更高（P<0.01）。HCMV感染主要定位于肿瘤上皮。HCMV阳性标本的Q-PCR结果表明，GC组织中的病毒拷贝数明显高于相邻正常标本（P<0.001）。临床统计分析表明，GC肿瘤组织中的HCMV载量与淋巴转移呈正相关（P=0.043），受试者操作特征（ROC）曲线下面积为0.6638。我们的数据清楚地显示了HCMV在GC患者中的流行情况。我们得出结论，恶性组织中的HCMV感染可能与GC的致癌作用或进展有关，并且可能与淋巴转移有关。
