Strati Irini F, Sinanoglou Vassilia J, Kora Lintita, Miniadis-Meimaroglou Sofia, Oreopoulou Vassiliki
Laboratory of Food Chemistry and Technology, School of Chemical Engineering, National Technical University of Athens, Iroon Polytechniou 5, Zografou 15780, Athens, Greece.
Instrumental Food Analysis Laboratory, Department of Food Technology, Technological Educational Institution of Athens, Ag. Spyridonos 12210 Egaleo, Greece.
Foods. 2012 Dec 19;1(1):52-65. doi: 10.3390/foods1010052.
Carotenoids are important antioxidant compounds, present in many foods of plant, animal and marine origin. The aim of the present study was to describe the carotenoid composition of tomato waste, prawn muscle and cephalothorax and avian (duck and goose) egg yolks through the use of a modified gradient elution HPLC method with a C reversed-phase column for the efficient separation and analysis of carotenoids and their -isomers. Elution time was reduced from 60 to 45 min without affecting the separation efficiency. All- lycopene predominated in tomato waste, followed by all--β-carotene, 13--lutein and all- lutein, while minor amounts of 9--lutein, 13--β-carotene and 9--β-carotene were also detected. Considering the above findings, tomato waste is confirmed to be an excellent source of recovering carotenoids, especially all- lycopene, for commercial use. Xanthophylls were the major carotenoids of avian egg yolks, all- lutein and all- zeaxanthin in duck and goose egg yolk, respectively. In the prawn, several carotenoids (zeaxanthin, all--lutein, canthaxanthin, cryptoxanthin, optical and geometrical astaxanthin isomers) were identified in considerable amounts by the same method. A major advantage of this HPLC method was the efficient separation of carotenoids and their -isomers, originating from a wide range of matrices.
类胡萝卜素是重要的抗氧化化合物，存在于许多植物性、动物性和海洋来源的食物中。本研究的目的是通过使用改良的梯度洗脱高效液相色谱法，采用C反相柱，对番茄渣、虾肌肉和头胸部以及禽（鸭和鹅）蛋黄中的类胡萝卜素组成进行描述，以实现类胡萝卜素及其异构体的高效分离和分析。洗脱时间从60分钟减少到45分钟，而不影响分离效率。番茄渣中全反式番茄红素占主导，其次是全反式β-胡萝卜素、13-顺式叶黄素和全反式叶黄素，同时还检测到少量的9-顺式叶黄素、13-顺式β-胡萝卜素和9-顺式β-胡萝卜素。考虑到上述发现，番茄渣被证实是回收类胡萝卜素（尤其是全反式番茄红素）用于商业用途的优质来源。叶黄素是禽蛋黄中的主要类胡萝卜素，鸭蛋黄和鹅蛋黄中分别为全反式叶黄素和全反式玉米黄质。在虾中，通过相同方法鉴定出了几种大量的类胡萝卜素（玉米黄质、全反式叶黄素、角黄素、隐黄质、光学和几何虾青素异构体）。这种高效液相色谱法的一个主要优点是能够有效分离来自多种基质的类胡萝卜素及其异构体。
