Proestos Charalampos, Varzakas Theo
. Laboratory of Food Chemistry, Department of Chemistry, National and Kapodistrian University of Athens, Athens 15771, Greece.
. Τechnological Educational Institute (TΕΙ) Peloponnese, Department of Food Technology, Antikalamos, Kalamata 24100, Greece.
Foods. 2017 Apr 4;6(4):28. doi: 10.3390/foods6040028.
The antioxidant properties and polyphenol content of some selected aromatic plants grown in Greece were studied. Plants were refluxed with 60% methanol after acid hydrolysis. The phenolic substances were quantified by High Performance Liquid Chromatography-Diode Array Detector (HPLC-DAD). The antioxidant capacity of the extracts was determined with the Rancimat test using sunflower oil as substrate. Free radical scavenging activity was measured using the stable free radical 1, 1-diphenyl-2-picrylhydrazyl (DPPH). Results were compared with standard butylated hydroxytoluene (BHT) and ascorbic acid. Total phenol concentration of the extracts was estimated with Folin-Ciocalteu reagent using gallic acid as standard. All plant extracts examined showed antioxidant capacity and contained phenolic compounds. Caffeic acid was detected in all the examined plant extracts. Ferulic acid was also detected in all the methanolic extracts, except from , in rather high concentration. The amount of total phenolics varied slightly in plant materials and ranged from 8.2 mg to 31.6 mg of gallic acid/g dry sample. The highest amount was found in , and the lowest in .
对希腊种植的一些选定芳香植物的抗氧化特性和多酚含量进行了研究。植物在酸水解后用60%甲醇回流。酚类物质通过高效液相色谱 - 二极管阵列检测器（HPLC - DAD）进行定量。提取物的抗氧化能力用Rancimat试验测定，以向日葵油为底物。使用稳定自由基1,1 - 二苯基 - 2 - 苦基肼（DPPH）测量自由基清除活性。结果与标准丁基羟基甲苯（BHT）和抗坏血酸进行比较。提取物的总酚浓度用福林 - 西奥尔特试剂以没食子酸为标准进行估计。所有检测的植物提取物均显示出抗氧化能力并含有酚类化合物。在所有检测的植物提取物中均检测到咖啡酸。除了[此处原文缺失植物名称]外，在所有甲醇提取物中也检测到阿魏酸，且浓度相当高。植物材料中总酚的含量略有不同，范围为8.2毫克至31.6毫克没食子酸/克干样品。在[此处原文缺失植物名称]中含量最高，在[此处原文缺失植物名称]中最低。
