Shaanxi Engineering Laboratory for Food Green Processing and Safety Control, College of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi'an 710119, China.
Food Funct. 2017 Mar 22;8(3):1282-1292. doi: 10.1039/c7fo00147a.
This study was designed to investigate the preventive effects of Red Fuji apple peel polyphenolic extract (APP) on vascular endothelial dysfunction and liver injury in mice fed a high choline diet. The mice were fed 3% dietary choline in drinking water for 8 weeks and displayed vascular dysfunction and liver damage (p < 0.01). The administration of APP at 600 and 900 mg per kg bw significantly elevated serum NO, HDL and 6-Keto-PGF1a levels and lowered serum TC, TG, LDL, ET-1 and TXB2 levels in the HC-fed mice. Besides, APP also caused the reduction of AST, ALT activities and MDA, CRP, TNF-α levels, and increased the hepatic GSH-Px and SOD activities of the HC-fed mice. Furthermore, the histopathology of the liver by conventional H&E and oil red O staining confirmed the liver steatosis induced by a choline diet and the hepatoprotective effect of APP. The experiment results indicated that the polyphenolic extract from apple peel might be regarded as a preventive and therapeutic product for the amelioration of HC diet-induced vascular dysfunction and hepatic injury.
本研究旨在探讨红富士苹果皮多酚提取物（APP）对高胆碱饮食诱导的小鼠血管内皮功能障碍和肝损伤的预防作用。小鼠饮用含 3%膳食胆碱的水 8 周，表现出血管功能障碍和肝损伤（p<0.01）。APP 以 600 和 900mg/kg bw 剂量给药可显著提高 HC 喂养小鼠的血清 NO、HDL 和 6-Keto-PGF1a 水平，并降低血清 TC、TG、LDL、ET-1 和 TXB2 水平。此外，APP 还可降低 AST、ALT 活性及 MDA、CRP、TNF-α水平，增加 HC 喂养小鼠肝组织 GSH-Px 和 SOD 活性。常规 H&E 和油红 O 染色的肝脏组织病理学证实了胆碱饮食诱导的肝脂肪变性和 APP 的保肝作用。实验结果表明，苹果皮中的多酚提取物可能作为一种预防和治疗产品，用于改善 HC 饮食诱导的血管功能障碍和肝损伤。
