McDonnell P J, McDonnell J M, Kessis T, Green W R, Shah K V
Wilmer Ophthalmological Institute, Johns Hopkins Hospital, Baltimore, MD 21205.
Hum Pathol. 1987 Nov;18(11):1115-9. doi: 10.1016/s0046-8177(87)80378-7.
Twenty-three conjunctival papillomas and 28 conjunctival dysplasias were examined for human papillomavirus (HPV)-DNA sequences by in situ hybridization with nick-translated 35S-labeled HPV probes. Adjacent paraffin sections were hybridized with HPV type 2, 6, 16, and 18 probes at Tm - 17 degrees C. Fifteen tissues, all papillomas, displayed positive hybridization with the HPV-6 probe. Infection with HPV-6 (or the closely related HPV-11) appeared to be responsible for most of the conjunctival papillomas of children and young adults. The presence of genital tract HPV-6 in these lesions suggests that some of the infections were acquired during passage through an infected birth canal. The lack of hybridization in adult conjunctival dysplasias indicates either that HPVs are not associated with this condition or that the probes and the technique utilized were not adequate for demonstration of this association.
通过用缺口平移法标记的35S标记的人乳头瘤病毒（HPV）探针进行原位杂交，检测了23例结膜乳头状瘤和28例结膜发育异常中的HPV-DNA序列。相邻的石蜡切片在比Tm低17℃的温度下与HPV 2型、6型、16型和18型探针杂交。15个组织（均为乳头状瘤）与HPV-6探针呈阳性杂交。HPV-6（或密切相关的HPV-11）感染似乎是儿童和年轻成人大多数结膜乳头状瘤的病因。这些病变中存在生殖道HPV-6表明部分感染是在通过受感染的产道时获得的。成人结膜发育异常中缺乏杂交信号表明要么HPV与这种情况无关，要么所使用的探针和技术不足以证明这种关联。
