Goodarzi Saied, Hadjiakhoondi Abbas, Yassa Narguess, Khanavi Mahnaz, Tofighi Zahra
Department of Pharmacognosy, Faculty of Pharmacy and Medicinal Plant Research Center, Tehran University of Medical Sciences, Tehran, Iran.
Iran J Pharm Res. 2016 Fall;15(4):901-906.
There are many efforts for identification of natural compounds from dietary or medicinal plants. Young roots and aerial parts of have been used as food additive or salad vegetable in some parts of Iran. In this study, different fractions of the root extract of were subjected for isolation and purification of secondary metabolites. The methanol extract of the roots was fractionated with hexane (HE), chloroform (CL), ethyl acetate (EA) and methanol (ME). Five novel compounds were isolated from HE, CL and EA using different chromatographic techniques and were identified by H-NMR, C-NMR, 2D-NMR and MS spectroscopic methods. Elucidated compounds with benzodioxole structure were characterized for the first time as 5-((propanoyl methyl)amino)-4,7-dimethoxybenzo[][1,3]dioxole (1), 5-(3-ethyloxiran-2-yloxy)-4,7-dimethoxybenzo[][1,3]dioxole (2), 4,7-dimethoxy-5-(propanonyl) benzo[][1,3]dioxole (3), 4-ethoxybenzo[][1,3]dioxol-6-carbaldehyde (4), and 4-(O-β-D-glucopyranosyl)-6-(3-propanyloxiran-2-yloxy)benzo[][1,3]dioxole (5).
人们为从食用或药用植物中鉴定天然化合物付出了诸多努力。在伊朗的一些地区，[植物名称]的幼根和地上部分被用作食品添加剂或沙拉蔬菜。在本研究中，对[植物名称]根提取物的不同馏分进行了次生代谢产物的分离和纯化。根的甲醇提取物用己烷（HE）、氯仿（CL）、乙酸乙酯（EA）和甲醇（ME）进行分馏。使用不同的色谱技术从HE、CL和EA中分离出五种新化合物，并通过氢核磁共振（H-NMR）、碳核磁共振（C-NMR）、二维核磁共振（2D-NMR）和质谱（MS）光谱方法进行鉴定。具有苯并二恶唑结构的已阐明化合物首次被表征为5-((丙酰基甲基)氨基)-4,7-二甲氧基苯并[][1,3]二恶唑（1）、5-(3-乙氧基环氧乙烷-2-基氧基)-4,7-二甲氧基苯并[][1,3]二恶唑（2）、4,7-二甲氧基-5-(丙酰基)苯并[][1,3]二恶唑（3）、4-乙氧基苯并[][1,3]二恶唑-6-甲醛（4）和4-(O-β-D-吡喃葡萄糖基)-6-(3-丙氧基环氧乙烷-2-基氧基)苯并[][1,3]二恶唑（5）。
