提取物根和果实部位的抗疟及细胞毒活性。
Antimalarial and cytotoxic activities of roots and fruits fractions of extract.
作者信息
Goodarzi Saied, Nateghpour Mehdi, Asgharian Parina, Hadjiakhoondi Abbas, Yassa Narguess, Tavakoli Saeed, Mirzaei Jalal, Farivar Leila, Haghi Afsaneh Motevalli, Tofighi Zahra
机构信息
Medicinal Plants Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
出版信息
Iran J Basic Med Sci. 2017 Dec;20(12):1318-1323. doi: 10.22038/IJBMS.2017.9554.
OBJECTIVES
(Apiaceae) is one of the two species of this genus which grows in different parts of Iran. Roots of this plant were rich in benzodioxoles and used as food additive or salad in Iran and near countries. The aim of present study was evaluation of antimalarial and cytotoxic effects of different fractions of fruits and roots extracts.
MATERIALS AND METHODS
Ripe fruits and roots of were extracted and fractionated by hexane, chloroform, ethyl acetate and methanol, separately. Antimalarial activities of fractions were performed based on suppressive test in mice model and percentage of parasitemia and suppression were determined for each sample. Cytotoxicity of fruits and roots fractions were investigated against human breast adenocarcinoma (MCF-7), colorectal carcinoma (SW480) and normal (L929) cell lines by MTT assay and IC of them were measured.
RESULTS
Hexane fraction of roots extract (RHE) and ethyl acetate fraction of fruits extract (FEA) of demonstrated highest parasite inhibition (73.3 and 72.3%, respectively at 500 mg/kg/day) which were significantly different from negative control group (<0.05). In addition, RHE showed potent anticancer activities against MCF-7 (IC of 0.01 µg/ml), SW480 (IC of 0.36 µg/ml) and L929 (IC of 0.70 µg/ml) cell lines.
CONCLUSION
According to the results, RHE and FEA fractions of could be introduced as excellent choice for antimalarial drug discovery. In addition, cytotoxic activity of RHE was noticeable.
目的
（伞形科）是该属生长在伊朗不同地区的两个物种之一。这种植物的根富含苯并二恶唑，在伊朗及周边国家被用作食品添加剂或沙拉。本研究的目的是评估果实和根提取物不同馏分的抗疟和细胞毒性作用。
材料与方法
分别用己烷、氯仿、乙酸乙酯和甲醇对成熟果实和根进行提取和分离。基于小鼠模型的抑制试验对馏分的抗疟活性进行检测，测定每个样品的疟原虫血症百分比和抑制率。通过MTT法研究果实和根馏分对人乳腺腺癌（MCF - 7）、结肠直肠癌（SW480）和正常（L929）细胞系的细胞毒性，并测定其半数抑制浓度（IC）。
结果
根提取物的己烷馏分（RHE）和果实提取物的乙酸乙酯馏分（FEA）表现出最高的寄生虫抑制率（分别在500 mg/kg/天的剂量下为73.3%和72.3%），与阴性对照组相比有显著差异（<0.05）。此外，RHE对MCF - 7（IC为0.01 µg/ml）、SW48（IC为0.36 µg/ml）和L929（IC为0.70 µg/ml）细胞系显示出较强的抗癌活性。
结论
根据结果，RHE和FEA馏分可作为抗疟药物研发的优秀选择。此外，RHE的细胞毒性活性显著。
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