人脂肪细胞的分化与代谢研究
Differentiation and Metabolic Interrogation of Human Adipocytes.
作者信息
Baker Nicki A, Muir Lindsey A, Lumeng Carey N, O'Rourke Robert W
机构信息
Section of General Surgery, Department of Surgery, University of Michigan Medical School, 2210 Taubman Center-5343, 1500 E. Medical Center Drive, Ann Arbor, MI, USA.
Department of Pediatrics and Communicable Diseases, University of Michigan Medical School, Ann Arbor, MI, USA.
出版信息
Methods Mol Biol. 2017;1566:61-76. doi: 10.1007/978-1-4939-6820-6_7.
Abstract
Adipocytes differentiated from preadipocytes provide a valuable model for the study of human adipocyte metabolism. We describe methods for isolation of human stromal vascular cells, expansion of preadipocytes, differentiation into mature adipocytes, and in vitro metabolic interrogation of adipocytes.
摘要
从脂肪前体细胞分化而来的脂肪细胞为研究人类脂肪细胞代谢提供了一个有价值的模型。我们描述了分离人类基质血管细胞、扩增脂肪前体细胞、分化为成熟脂肪细胞以及对脂肪细胞进行体外代谢研究的方法。
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