Wright N E, Gilbert P
Department of Pharmacy, University of Manchester, UK.
J Antimicrob Chemother. 1987 Sep;20(3):303-12. doi: 10.1093/jac/20.3.303.
The sensitivity of Escherichia coli to the lytic action of polymyxin B was assessed for cells grown in a chemostat at a variety of specific growth rates and under conditions of carbon, nitrogen, phosphorus and magnesium limitation. Magnesium and phosphorus limited cells demonstrated a trend of increased resistance with increasing growth-rate, whereas carbon and nitrogen limited cells increased their sensitivity as the growth rate increased. Divergent patterns of sensitivity, such as these, allowed a number of models for resistance towards polymyxin to be assessed. It was not possible to attribute polymyxin sensitivity to any single envelope component; rather the patterns of sensitivity reflect, in a complex manner, presence of envelope proteins and acidic phospholipids.
在恒化器中以各种特定生长速率培养的大肠杆菌细胞，在碳、氮、磷和镁限制条件下，评估了其对多粘菌素B裂解作用的敏感性。镁和磷限制的细胞表现出随着生长速率增加而抗性增加的趋势，而碳和氮限制的细胞则随着生长速率增加而敏感性增加。诸如此类不同的敏感性模式，使得可以评估多种多粘菌素抗性模型。不可能将多粘菌素敏感性归因于任何单一的包膜成分；相反，敏感性模式以复杂的方式反映了包膜蛋白和酸性磷脂的存在。
