离子载体A23187对多粘菌素B九肽致敏大肠杆菌细胞内镁含量的调控
Manipulation of intracellular magnesium content in polymyxin B nonapeptide-sensitized Escherichia coli by ionophore A23187.
作者信息
Alatossava T, Jütte H, Kuhn A, Kellenberger E
出版信息
J Bacteriol. 1985 Apr;162(1):413-9. doi: 10.1128/jb.162.1.413-419.1985.
Abstract
Escherichia coli B cells were sensitized to ionophore A23187 by polymyxin B nonapeptide, and the induced magnesium and potassium ion fluxes were studied. Combined ionophore treatment permeabilized the cytoplasmic membrane of E. coli in an ion-specific manner and allowed the manipulation of intracellular Mg2+ content from the outside. A23187-induced Mg2+ efflux or influx was dependent on the free Mg2+ concentration gradient between the outside and inside of the cytoplasmic membrane and on the pH gradient. Most of the intracellular Mg2+ was bound, whereas only 1 to 2 mM was free in solution in the cellular sap.
摘要
用多粘菌素B九肽使大肠杆菌B细胞对离子载体A23187敏感，并研究诱导的镁离子和钾离子通量。联合离子载体处理以离子特异性方式使大肠杆菌的细胞质膜透化，并允许从外部操纵细胞内Mg2+含量。A23187诱导的Mg2+外流或内流取决于细胞质膜内外的游离Mg2+浓度梯度以及pH梯度。细胞内的大部分Mg2+是结合的，而细胞液中溶液中游离的只有1至2 mM。
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