三个外显子的差异使用产生了至少五种不同的编码人白细胞共同抗原的mRNA。
Differential usage of three exons generates at least five different mRNAs encoding human leukocyte common antigens.
作者信息
Streuli M, Hall L R, Saga Y, Schlossman S F, Saito H
机构信息
Division of Tumor Immunology, Dana-Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts 02115.
出版信息
J Exp Med. 1987 Nov 1;166(5):1548-66. doi: 10.1084/jem.166.5.1548.
Abstract
Leukocyte common antigens (LCAs, also known as T200 and CD 45) are integral membrane proteins expressed exclusively on hematopoietic cells. These molecules exhibit varying molecular masses and epitopes when expressed in different cell types. To determine the genetic bases for the generation of this diversity, three classes of human LCA cDNA clones that are different near their 5' ends have been isolated. These differences arose as a result of differential usage of three exons as determined from an analysis of a genomic DNA clone. Furthermore, Northern blot analysis with LCA exon-specific probes demonstrates the existence of at least two more LCA mRNA forms that are generated by differential splicing. A comparison of the human and mouse LCA protein sequences revealed a marked difference only in the extracellular domain.
摘要
白细胞共同抗原（LCAs，也称为T200和CD 45）是仅在造血细胞上表达的整合膜蛋白。这些分子在不同细胞类型中表达时表现出不同的分子量和表位。为了确定这种多样性产生的遗传基础，已分离出三类在其5'端附近不同的人LCA cDNA克隆。根据对基因组DNA克隆的分析确定，这些差异是由于三个外显子的不同使用造成的。此外，用LCA外显子特异性探针进行的Northern印迹分析表明，至少存在另外两种由差异剪接产生的LCA mRNA形式。人和小鼠LCA蛋白序列的比较仅在细胞外结构域显示出明显差异。
相似文献
1
Differential usage of three exons generates at least five different mRNAs encoding human leukocyte common antigens.三个外显子的差异使用产生了至少五种不同的编码人白细胞共同抗原的mRNA。
J Exp Med. 1987 Nov 1;166(5):1548-66. doi: 10.1084/jem.166.5.1548.
2
Identification of the sequence required for expression of the 2H4 epitope on the human leukocyte common antigens.鉴定人类白细胞共同抗原上2H4表位表达所需的序列。
J Exp Med. 1987 Nov 1;166(5):1567-72. doi: 10.1084/jem.166.5.1567.
3
Complete exon-intron organization of the human leukocyte common antigen (CD45) gene.人类白细胞共同抗原（CD45）基因完整的外显子-内含子结构
J Immunol. 1988 Oct 15;141(8):2781-7.
4
Integrity of the exon 6 sequence is essential for tissue-specific alternative splicing of human leukocyte common antigen pre-mRNA.外显子6序列的完整性对于人白细胞共同抗原前体mRNA的组织特异性可变剪接至关重要。
Mol Cell Biol. 1989 Oct;9(10):4550-5. doi: 10.1128/mcb.9.10.4550-4555.1989.
5
Evidence from cDNA clones that the rat leukocyte-common antigen (T200) spans the lipid bilayer and contains a cytoplasmic domain of 80,000 Mr.来自cDNA克隆的证据表明，大鼠白细胞共同抗原（T200）跨越脂质双层，包含一个80,000道尔顿的胞质结构域。
Cell. 1985 May;41(1):83-93. doi: 10.1016/0092-8674(85)90063-7.
6
Regulation of tissue-specific alternative splicing: exon-specific cis-elements govern the splicing of leukocyte common antigen pre-mRNA.组织特异性可变剪接的调控：外显子特异性顺式元件控制白细胞共同抗原前体mRNA的剪接。
EMBO J. 1989 Mar;8(3):787-96. doi: 10.1002/j.1460-2075.1989.tb03439.x.
7
Cloned murine T200 (Ly-5) cDNA reveals multiple transcripts within B- and T-lymphocyte lineages.克隆的小鼠T200（Ly-5）cDNA揭示了B淋巴细胞和T淋巴细胞谱系中的多种转录本。
Proc Natl Acad Sci U S A. 1987 Jan;84(1):161-5. doi: 10.1073/pnas.84.1.161.
8
Identification of the alternatively spliced exons of murine CD45 (T200) required for reactivity with B220 and other T200-restricted antibodies.鉴定与B220及其他T200限制性抗体发生反应所需的小鼠CD45（T200）可变剪接外显子。
J Exp Med. 1989 Mar 1;169(3):1179-84. doi: 10.1084/jem.169.3.1179.
9
B-cell variant of mouse T200 (Ly-5): evidence for alternative mRNA splicing.小鼠T200（Ly-5）的B细胞变体：选择性mRNA剪接的证据。
Proc Natl Acad Sci U S A. 1987 Aug;84(15):5360-3. doi: 10.1073/pnas.84.15.5360.
10
Sequence conservation in potential regulatory regions of the mouse and human leukocyte common antigen gene.小鼠和人类白细胞共同抗原基因潜在调控区域中的序列保守性。
J Biol Chem. 1989 Apr 15;264(11):6220-9.
引用本文的文献
1
Restricted human CD45 isoglycoforms serve as functional E-selectin ligands and delineate hematopoietic maturity.受限的人类CD45同工型作为功能性E-选择素配体并描绘造血成熟度。
J Biol Chem. 2025 Jul 1;301(8):110431. doi: 10.1016/j.jbc.2025.110431.
2
Delineation of chicken immune markers in the era of omics and multicolor flow cytometry.组学与多色流式细胞术时代鸡免疫标志物的描绘
Front Vet Sci. 2024 May 23;11:1385400. doi: 10.3389/fvets.2024.1385400. eCollection 2024.
3
The HTLV-1 viral oncoproteins Tax and HBZ reprogram the cellular mRNA splicing landscape.HTLV-1 病毒癌蛋白 Tax 和 HBZ 重编程细胞 mRNA 剪接景观。
PLoS Pathog. 2021 Sep 20;17(9):e1009919. doi: 10.1371/journal.ppat.1009919. eCollection 2021 Sep.
4
The three as: Alternative splicing, alternative polyadenylation and their impact on apoptosis in immune function.三种方式：选择性剪接、选择性多聚腺苷酸化及其对免疫功能中细胞凋亡的影响。
Immunol Rev. 2021 Nov;304(1):30-50. doi: 10.1111/imr.13018. Epub 2021 Aug 8.
5
Allelic Variation in Protein Tyrosine Phosphatase Receptor Type-C in Cattle Influences Erythrocyte, Leukocyte and Humoral Responses to Infestation With the Cattle Tick .蛋白酪氨酸磷酸酯酶受体 C 型在牛中的等位基因变异影响其对牛蜱侵染的红细胞、白细胞和体液反应。
Front Immunol. 2021 Jul 9;12:675979. doi: 10.3389/fimmu.2021.675979. eCollection 2021.
6
The Roseoloviruses Downregulate the Protein Tyrosine Phosphatase PTPRC (CD45).玫瑰疹病毒下调蛋白酪氨酸磷酸酶 PTPRC（CD45）。
J Virol. 2021 Jun 24;95(14):e0162820. doi: 10.1128/JVI.01628-20.
7
Transient antibody targeting of CD45RC induces transplant tolerance and potent antigen-specific regulatory T cells.CD45RC 瞬时抗体靶向诱导移植耐受和强效抗原特异性调节性 T 细胞。
JCI Insight. 2017 Feb 9;2(3):e90088. doi: 10.1172/jci.insight.90088.
8
Overexpression of CD45RA isoforms in carriers of the C77G mutation leads to hyporeactivity of CD4+CD25highFoxp3+ regulatory T cells.C77G 突变携带者中 CD45RA 同工型的过度表达导致 CD4+CD25highFoxp3+调节性 T 细胞反应性降低。
Genes Immun. 2015 Dec;16(8):519-27. doi: 10.1038/gene.2015.39. Epub 2015 Sep 10.
9
Viral interference with functions of the cellular receptor tyrosine phosphatase CD45.病毒对细胞受体酪氨酸磷酸酶CD45功能的干扰。
Viruses. 2015 Mar 23;7(3):1540-57. doi: 10.3390/v7031540.
10
A higher risk of acute rejection of human kidney allografts can be predicted from the level of CD45RC expressed by the recipients' CD8 T cells.受者 CD8 T 细胞表达的 CD45RC 水平可预测人肾同种异体移植物急性排斥反应的风险增加。
PLoS One. 2013 Jul 24;8(7):e69791. doi: 10.1371/journal.pone.0069791. Print 2013.
本文引用的文献
1
Disposition of T200 glycoprotein in the plasma membrane of a murine lymphoma cell line.T200糖蛋白在小鼠淋巴瘤细胞系质膜中的分布
J Biol Chem. 1980 Feb 25;255(4):1662-9.
2
A simple method for displaying the hydropathic character of a protein.一种展示蛋白质亲水性特征的简单方法。
J Mol Biol. 1982 May 5;157(1):105-32. doi: 10.1016/0022-2836(82)90515-0.
3
A catalogue of splice junction sequences.剪接连接序列目录。
Nucleic Acids Res. 1982 Jan 22;10(2):459-72. doi: 10.1093/nar/10.2.459.
4
Structural features and selective expression of three Ly-5+ cell-surface molecules.三种Ly-5+细胞表面分子的结构特征与选择性表达
Immunogenetics. 1981;14(1-2):101-6. doi: 10.1007/BF00344303.
5
The differentiation and function of human T lymphocytes.人类T淋巴细胞的分化与功能。
Cell. 1980 Apr;19(4):821-7. doi: 10.1016/0092-8674(80)90072-0.
6
A human leukocyte antigen identified by a monoclonal antibody.一种由单克隆抗体识别的人类白细胞抗原。
Hum Immunol. 1981 Nov;3(3):231-45. doi: 10.1016/0198-8859(81)90020-3.
7
Human homologue of murine T200 glycoprotein.鼠T200糖蛋白的人类同源物。
J Exp Med. 1980 Oct 1;152(4):842-52. doi: 10.1084/jem.152.4.842.
8
Evidence for the occurrence of O-glycosidically linked oligosaccharides of poly-N-acetyllactosamine type on the human leucocyte common antigen.关于人白细胞共同抗原上存在多聚-N-乙酰乳糖胺型O-糖苷连接寡糖的证据。
Biochem Biophys Res Commun. 1983 Jan 27;110(2):424-31. doi: 10.1016/0006-291x(83)91166-x.
9
10
Human epidermal growth factor receptor cDNA sequence and aberrant expression of the amplified gene in A431 epidermoid carcinoma cells.人表皮生长因子受体cDNA序列及扩增基因在A431表皮样癌细胞中的异常表达。
Nature. 1984;309(5967):418-25. doi: 10.1038/309418a0.
Zotero 插件