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隔药灸对葡聚糖硫酸钠诱导的溃疡性结肠炎大鼠结肠微小RNA表达谱的影响

Effects of Herb-Partitioned Moxibustion on the miRNA Expression Profiles in Colon from Rats with DSS-Induced Ulcerative Colitis.

作者信息

Huang Yan, Ma Zhe, Cui Yun-Hua, Dong Hong-Sheng, Zhao Ji-Meng, Dou Chuan-Zi, Liu Hui-Rong, Li Jing, Wu Huan-Gan

机构信息

Key Laboratory of Acupuncture and Immunological Effects, Shanghai University of Traditional Chinese Medicine, Shanghai 200030, China; Shanghai Research Institute of Acupuncture and Meridian, Shanghai University of Traditional Chinese Medicine, Shanghai 200030, China.

Yueyang Clinical Medical College, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China.

出版信息

Evid Based Complement Alternat Med. 2017;2017:1767301. doi: 10.1155/2017/1767301. Epub 2017 Jan 26.

DOI:10.1155/2017/1767301
PMID:28246536
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5299174/
Abstract

This study explored the mechanism of herb-partitioned moxibustion (HM) on dextran sulfate sodium- (DSS-) induced ulcerative colitis (UC) from the miRNA perspective. Rats were randomly divided into 3 groups [normal control (NC) group, UC model (UC) group, and herb-partitioned moxibustion (UCHM) group]. The UC and UCHM groups were administered 4% DSS for 7 days. The UCHM group received HM at the Tianshu (bilateral, ST25). The effect of HM on UC was observed and the miRNA expression profile in the colon tissues was analyzed. Compared with the UC group, the body weights were significantly higher in the UCHM group on day 14 ( < 0.001); the macroscopic colon injury scores and microscopic histopathology scores in the UCHM group decreased ( < 0.05); and there were 15 differentially expressed miRNAs in the UCHM group. The changes in miR-184 and miR-490-5p expression levels on the UC were reversed by HM intervention. Validation using qRT-PCR showed that two miRNAs expression trend was consistent with the sequencing results. HM at ST25 might regulate miR-184 and miR-490-5p expression, act on the transcription of their target genes to regulate inflammatory signaling pathways, and attenuate inflammation and tissue injury in the colons of rats with DSS-induced UC.

摘要

本研究从微小RNA（miRNA）角度探讨隔物灸（HM）对葡聚糖硫酸钠（DSS）诱导的溃疡性结肠炎（UC）的作用机制。将大鼠随机分为3组[正常对照组（NC组）、UC模型组（UC组）和隔物灸组（UCHM组）]。UC组和UCHM组给予4% DSS，持续7天。UCHM组于天枢穴（双侧，ST25）进行隔物灸。观察隔物灸对UC的影响，并分析结肠组织中的miRNA表达谱。与UC组相比，UCHM组在第14天体重显著升高（<0.001）；UCHM组的宏观结肠损伤评分和微观组织病理学评分降低（<0.05）；UCHM组有15个差异表达的miRNA。HM干预可逆转UC中miR-184和miR-490-5p表达水平的变化。qRT-PCR验证表明，两种miRNA的表达趋势与测序结果一致。ST25处的隔物灸可能通过调节miR-184和miR-490-5p的表达，作用于其靶基因的转录来调节炎症信号通路，减轻DSS诱导的UC大鼠结肠的炎症和组织损伤。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bb51/5299174/d1087612d925/ECAM2017-1767301.009.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bb51/5299174/d1087612d925/ECAM2017-1767301.009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bb51/5299174/abe70e44b710/ECAM2017-1767301.001.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bb51/5299174/d1087612d925/ECAM2017-1767301.009.jpg

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