Liu J C, Tian Y, Qiu F X, Li F, Hao Y P, Zhang X S
Institute of Epidemiology and Microbiology, Chinese Academy of Preventive Medicine, Beijing, People's Republic of China.
J Med Virol. 1987 Oct;23(2):175-7. doi: 10.1002/jmv.1890230210.
This article reports for the first time in China the results of serotyping and subgrouping of some rotavirus strains obtained from faecal fluids of cases with infant diarrhoea. Infant diarrhoea rotavirus serotype 1 (Wa strain)-specific and serotype 2 (KUN strain)-specific hyperimmune sera, and infant diarrhoea rotavirus serotype 3-specific, rotavirus subgroup I-specific and subgroup II-specific monoclonal antibodies were used in serotyping and subgrouping by means of enzyme-linked immunosorbent assay of 14 rotavirus strains which had been identified by electron microscopy. Results showed that three out of four Beijing strains in 1982 belonged to serotype 2 and subgroup I rotavirus, one belonged to serotype 3 and subgroup II rotavirus; one out of nine Beijing strains in 1984 belonged to serotype 1 and subgroup II rotavirus, seven belonged to serotype 2 and subgroup I rotavirus, one belonged to serotype 3 and subgroup II rotavirus; and one Kunming strain in 1984 belonged to serotype 3 and subgroup II rotavirus.
本文首次在中国报道了从婴儿腹泻病例粪便中获取的部分轮状病毒株的血清分型和亚组分型结果。采用婴儿腹泻轮状病毒1型（Wa株）特异性和2型（KUN株）特异性超免疫血清，以及婴儿腹泻轮状病毒3型特异性、轮状病毒I亚组特异性和II亚组特异性单克隆抗体，通过酶联免疫吸附试验对14株经电子显微镜鉴定的轮状病毒进行血清分型和亚组分型。结果显示，1982年的4株北京株中，3株属于2型轮状病毒和I亚组，1株属于3型轮状病毒和II亚组；1984年的9株北京株中，1株属于1型轮状病毒和II亚组，7株属于2型轮状病毒和I亚组，1株属于3型轮状病毒和II亚组；1984年的1株昆明株属于3型轮状病毒和II亚组。
