Zhang Fang, Zhang Jian-Guo, Qu Jie, Zhang Qi, Prasad Chandan, Wei Zhao-Jun
School of Food Science and Engineering, Hefei University of Technology, Hefei 230009, People's Republic of China.
Department of Nutrition and Food Sciences, Texas Woman's University, Denton, TX, USA.
Food Chem Toxicol. 2017 Nov;109(Pt 2):910-922. doi: 10.1016/j.fct.2017.02.038. Epub 2017 Feb 27.
The anti-cancerous activity of 6-gingerol extracted from Tongling White Ginger was investigated. 6-Gingerol inhibited the growth of HeLa cells with IC50 (96.32 μM) and IC80 (133.01 μM) and led to morphological changes, induced the cell cycle arrest in G0/G1-phase and ultimately resulted into apoptosis. Among cell cycle-related genes and proteins, the expression of cyclin (A, D1, E1) reduced, while of CDK-1, p21 and p27 showed slight decrease, except cyclin B1 and E1 (protein). Western blotting reported the induction of apoptosis with an increased Bax/Bcl-2 ratio, release of cytochrome c, cleavage of caspase-3, -8, -9 and PRPP in treated cells. 6-Gingerol activated AMPK, but inhibited PI3K/AKT phosphorylation with reduced P70S6K expression and also suppressed the mTOR phosphorylation. 6-Gingerol with 5-FU and Ptx resulted in 83.2% and 52% inhibition respectively, this synergy have stimulated apoptosis proteins more efficiently as compared to 6-Gingerol alone (10.75%) under in vitro conditions.
研究了从铜陵白姜中提取的6-姜酚的抗癌活性。6-姜酚以IC50（96.32μM）和IC80（133.01μM）抑制HeLa细胞的生长，并导致形态变化，诱导细胞周期停滞在G0/G1期，最终导致细胞凋亡。在细胞周期相关基因和蛋白质中，细胞周期蛋白（A、D1、E1）的表达降低，而CDK-1、p21和p27的表达略有下降，但细胞周期蛋白B1和E1（蛋白质）除外。蛋白质印迹法显示，处理后的细胞中Bax/Bcl-2比值增加、细胞色素c释放、半胱天冬酶-3、-8、-9和PRPP裂解，从而诱导细胞凋亡。6-姜酚激活AMPK，但抑制PI3K/AKT磷酸化，降低P70S6K表达，并抑制mTOR磷酸化。6-姜酚与5-氟尿嘧啶和紫杉醇联合使用时，分别产生83.2%和52%的抑制率，与单独使用6-姜酚（10.75%）相比，这种协同作用在体外条件下更有效地刺激了凋亡蛋白的产生。
