Fastbom J, Pazos A, Probst A, Palacios J M
Preclinical Research, Sandoz Ltd., Basle, Switzerland.
Neuroscience. 1987 Sep;22(3):827-39. doi: 10.1016/0306-4522(87)92962-9.
The distribution of adenosine A1 receptors in the human brain was studied by autoradiography in post mortem brain tissues from 26 subjects without reported neurological disease. N6-[3H]Cyclohexyl-adenosine was used as the ligand. For comparison, adjacent sections of some regions were examined histochemically for 5'-nucleotidase activity. The receptor sites were heterogeneously distributed throughout the CNS. The highest receptor densities were found in the stratum oriens, pyramidale and radiatum of the hippocampus. High densities were also found in the cerebral cortex and the striatum. In the thalamus there was a heterogeneous distribution of binding sites with a high density in structures such as the medial and anterior nucleus. Intermediate receptor densities were found in the accumbens, the olfactory tubercle and most parts of the amygdala among others. The hypothalamus had low receptor densities. In the brainstem and the spinal cord very low receptor concentrations were found. However, in some structures such as the substantia nigra, the colliculus superior and the substantia gelatinosa of the spinal cord a low level of binding could be measured. The cerebellar cortex showed low densities of receptors. Structures showing high levels of 5'-nucleotidase activity were the hippocampus, the striatum and parts of the cerebral cortex among other regions. In general there was a poor correlation between the localization of A1 receptors and the 5'-nucleotidase activity. Some regions, however, showed a similar distribution of these two markers. In general, the distribution of adenosine A1 receptors found in the human brain is comparable to that found in previous autoradiographic studies in the rat brain. However, some regional differences were observed in, for example, the cerebral cortex, the striatum and the cerebellar cortex. These differences may prove to be functionally relevant.
通过放射自显影术，对26名无神经疾病报告的受试者的死后脑组织进行研究，以观察人脑中腺苷A1受体的分布。使用N6-[3H]环己基腺苷作为配体。为作比较，对部分区域的相邻切片进行了5'-核苷酸酶活性的组织化学检查。受体位点在整个中枢神经系统中呈异质性分布。在海马体的 Oriens层、锥体层和辐射层中发现受体密度最高。在大脑皮层和纹状体中也发现了高密度。在丘脑中，结合位点分布不均，在内侧核和前核等结构中密度较高。在伏隔核、嗅结节和杏仁核的大部分区域等发现中等受体密度。下丘脑的受体密度较低。在脑干和脊髓中发现受体浓度非常低。然而，在某些结构如黑质、上丘和脊髓的胶状质中可检测到低水平的结合。小脑皮层显示受体密度较低。显示高水平5'-核苷酸酶活性的结构包括海马体、纹状体和大脑皮层的部分区域等。总体而言，A1受体的定位与5'-核苷酸酶活性之间的相关性较差。然而，某些区域显示这两种标记物的分布相似。一般来说，在人脑中发现的腺苷A1受体分布与先前在大鼠脑中进行的放射自显影研究中发现的分布相当。然而，在例如大脑皮层、纹状体和小脑皮层中观察到了一些区域差异。这些差异可能在功能上具有相关性。
