Department of Biochemistry and Molecular Biology, Pondicherry University, Puducherry 605 014, India.
Department of Chemistry, Periyar University, Salem 636011, India.
Mater Sci Eng C Mater Biol Appl. 2017 May 1;74:597-608. doi: 10.1016/j.msec.2017.02.003. Epub 2017 Feb 7.
Biomedical application of selenium nanoparticles (SeNPs) demands the eco-friendly composite for synthesis of SeNPs. The present study reports an aqueous extract of Allium sativum (AqEAS) plug-up the current need. Modern spectroscopic, microscopic and gravimetric techniques were employed to characterize the synthesized nanoparticles. Characterization studies revealed the formation of crystalline spherical shaped SeNPs. FTIR spectrum brings out the presence of different functional groups in AqEAS, which influence the SeNPs formation and stabilization. Furthermore the different aspects of the interaction between SeNPs and CT-DNA were scrutinized by various spectroscopic and cyclic voltametric studies. The results reveals the intercalation and groove binding mode of interaction of SeNPs with stacked base pair of CT-DNA. The Stern-Volmer quenching constant (K) were found to be 7.02×10M- (ethidium bromide), 4.22×10 M- (acridine orange) and 7.6×10M- (Hoechst) indicating strong binding of SeNPs with CT-DNA. The SeNPs - CT-DNA interactions were directly visualized by atomic force microscopy. The present study unveils the cost effective, innocuous, highly stable SeNPs intricate mechanism of DNA interaction, which will be a milestone in DNA targeted chemotherapy.
硒纳米粒子（SeNPs）的生物医学应用需要合成 SeNPs 的环保复合材料。本研究报道了一种大蒜（AqEAS）的水提物可以满足当前的需求。现代光谱学、显微镜和重量法技术被用于表征合成的纳米粒子。表征研究表明，形成了结晶的球形 SeNPs。傅里叶变换红外（FTIR）光谱显示出 AqEAS 中存在不同的功能基团，这些基团影响 SeNPs 的形成和稳定。此外，通过各种光谱和循环伏安研究深入研究了 SeNPs 与 CT-DNA 之间的不同相互作用方面。结果表明，SeNPs 与 CT-DNA 的堆积碱基对之间存在嵌入和沟结合相互作用模式。发现 Stern-Volmer 猝灭常数（K）分别为 7.02×10M-（溴化乙锭）、4.22×10 M-（吖啶橙）和 7.6×10M-（Hoechst），表明 SeNPs 与 CT-DNA 具有强结合能力。原子力显微镜直接观察到了 SeNPs-CT-DNA 相互作用。本研究揭示了成本效益高、无毒、高度稳定的 SeNPs 与 DNA 相互作用的复杂机制，这将是 DNA 靶向化疗的一个里程碑。
