Wachtel D, Thieler H, Lenhardt E
Institut für Medizinische Mikrobiologie, Medizinischen Akademie Erfurt.
Z Urol Nephrol. 1987 Aug;80(8):491-4.
Dialysates for the haemodialysis are produced unsterile and usually contain bacteria. Own investigations of bicarbonate dialysate and adequate initial solutions comprised sterility tests, determinations of the germ count and germ tolerance experiments. Only the "acid concentrate" was sterile. In the other solutions Corynebacteria, Acinetobacter and Pseudomonas bacteria dominated as typical water germs. In the fresh reverse osmosis water and the bicarbonate dialysate as well as in the recently produced 35-mmolar and 1-molar NaHCO3-solution the germ count was in each case about 10(5)/l and did not change itself essentially at room temperature within 6 hours. The "acid concentrate" and at a lower level also the 1-molar NaHCO3-concentrate have an antibacterial effect. The reverse osmosis water is the main contamination source for the bicarbonate dialysate, the application of which within 6 hours seems worth being used on account of the low germ count.
用于血液透析的透析液在生产时未进行灭菌处理，通常含有细菌。我们对碳酸氢盐透析液和合适的初始溶液进行了自身研究，包括无菌测试、细菌计数测定和细菌耐受性实验。只有“酸浓缩液”是无菌的。在其他溶液中，棒状杆菌、不动杆菌和假单胞菌作为典型的水生细菌占主导地位。在新鲜的反渗透水、碳酸氢盐透析液以及最近生产的35毫摩尔和1摩尔的NaHCO₃溶液中，细菌计数在每种情况下约为10⁵/升，并且在室温下6小时内基本没有变化。“酸浓缩液”以及较低程度上的1摩尔NaHCO₃浓缩液具有抗菌作用。反渗透水是碳酸氢盐透析液的主要污染源，鉴于其细菌计数较低，在6小时内使用该透析液似乎是值得的。
