Ruderman M A, Yungbluth M M, del Greco F, Krumlovsky F A, Beske R G
Dialysis Center, Northwestern Memorial Hospital, Chicago, IL 60611.
ASAIO Trans. 1988 Jul-Sep;34(3):606-7.
To determine whether bacteriologic hazards associated with bicarbonate concentrate may be reduced or prevented, evaluation of a system consisting of a mixing tank supplied with R.O. water and two separate storage tanks was undertaken. Bicarbonate was mixed on a schedule specific to usage and dispensed to the storage tanks from which it was drawn off into 2.5 gallon jugs for each treatment. The mixing tank was disinfected with hypochlorite solution (200 ppm) before each use. Storage tanks and 2.5 gallon jugs were disinfected weekly and sampled immediately before scheduled disinfection and after the longest storage time. The mixing tank was sampled for corresponding batches. A 1 ml inoculum of concentrate was plated onto tryptic soy sheep blood agar, incubated for 48 hours at 35 degrees C and colony counts/ml recorded. A total of 225 batches on a 10% sampling schedule were monitored. All cultures have grown less than 50 cfu/ml, which is less than AAMI standards for maximum allowable level for water and dialysate. Bacteriologically safe bicarbonate concentrate can be reliably produced using R.O. water, controlled storage times, and rigid disinfection schedules.
为了确定与碳酸氢盐浓缩液相关的细菌学危害是否可以降低或预防，对一个由配备反渗透水的混合罐和两个独立储存罐组成的系统进行了评估。碳酸氢盐按照特定的使用时间表进行混合，并分配到储存罐中，然后从储存罐中抽取到每个处理用的2.5加仑容器中。每次使用前，混合罐用次氯酸盐溶液（200 ppm）进行消毒。储存罐和2.5加仑容器每周消毒一次，并在预定消毒前和最长储存时间后立即取样。混合罐对相应批次进行取样。将1毫升浓缩液接种物接种到胰蛋白酶大豆羊血琼脂上，在35℃下培养48小时，并记录每毫升菌落数。按照10%的抽样计划共监测了225批次。所有培养物的生长均低于50 cfu/ml，这低于美国医疗器械促进协会（AAMI）对水和透析液最大允许水平的标准。使用反渗透水、控制储存时间和严格的消毒时间表，可以可靠地生产出细菌学安全的碳酸氢盐浓缩液。
