Yabe Y, Kuramitsu M
Department of Virology, Okayama University Medical School, Japan.
Acta Med Okayama. 1987 Oct;41(5):233-5. doi: 10.18926/AMO/31756.
A rapid method was devised for the detection of virus particles in wart specimens. The upper layer of warts was cut perpendicularly to the surface, and the freshly cut surface was lightly touched to an electron microscope grid. The grid was then stained with a small drop of phosphotungstate and observed electron microscopically. On the specimen grid thus prepared, papillomavirus particles were easily discriminated from tissue debris. Papillomavirus particles were detected in 71% of verrucae plantares, 78% of verrucae palmares, 50% of verrucae vulgares and 75% of condylomata acuminata by the present method.
设计了一种快速检测疣标本中病毒颗粒的方法。将疣的上层垂直于表面切开，将新切开的表面轻轻接触电子显微镜网格。然后用一小滴磷钨酸盐对网格进行染色，并在电子显微镜下观察。在这样制备的标本网格上，乳头瘤病毒颗粒很容易与组织碎片区分开来。通过本方法，在71%的跖疣、78%的掌疣、50%的寻常疣和75%的尖锐湿疣中检测到乳头瘤病毒颗粒。
