Bioresource Utilization Laboratory, College of Engineering, China Agricultural University, Beijing 100083, PR China; William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, 151 West Woodruff Ave., Columbus, OH 43210, USA.
William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, 151 West Woodruff Ave., Columbus, OH 43210, USA.
Bioresour Technol. 2017 Jun;233:51-57. doi: 10.1016/j.biortech.2017.02.079. Epub 2017 Feb 21.
The production of n-butanol from sugarcane juice by metabolically engineered Clostridium tyrobutyricum Ct(Δack)-pscrBAK overexpressing scr operon genes (scrB, scrA, and scrK) for sucrose catabolism and an aldehyde/alcohol dehydrogenase gene (adhE2) for butanol biosynthesis was studied with corn steep liquor (CSL) as a low-cost nitrogen source. In free cell fermentation, butanol production of ∼16g/L at a yield of 0.31±0.02g/g and productivity of 0.33±0.02g/L·h was obtained from sucrose and yield of 0.24±0.02g/g and productivity of 0.30±0.01g/L·h from sugarcane juice containing sucrose, glucose and fructose. The fermentation was also studied in a fibrous bed bioreactor (FBB) operated in a repeated batch mode for 10 consecutive cycles in 10days, achieving an average butanol yield of 0.21±0.02g/g and productivity of 0.53±0.05g/L·h from sugarcane juice, demonstrating its long-term stability without applying the antibiotic selection pressure.
利用玉米浆（CSL）作为低成本氮源，研究了代谢工程化的丁酸梭菌 Clostridium tyrobutyricum Ct(Δack)-pscrBAK 过表达蔗糖分解代谢 scr 操纵基因（scrB、scrA 和 scrK）和醛/醇脱氢酶基因（adhE2），从甘蔗汁中生产 n-丁醇。在游离细胞发酵中，从蔗糖中获得了约 16g/L 的丁醇产量，产率为 0.31±0.02g/g，生产力为 0.33±0.02g/L·h；从含有蔗糖、葡萄糖和果糖的甘蔗汁中获得了 0.24±0.02g/g 的产率和 0.30±0.01g/L·h 的生产力。还在纤维床生物反应器（FBB）中进行了发酵研究，在 10 天内以重复分批模式运行了 10 个连续周期，从甘蔗汁中获得了平均 0.21±0.02g/g 的丁醇产率和 0.53±0.05g/L·h 的生产力，证明了其在不施加抗生素选择压力的情况下具有长期稳定性。
