Tian X J, Wang X C, Ye B, Li C L, Zhang Y, Ma L
School of Health Sciences, Wuhan University, Wuhan 430071, China.
Reproductive Medicine Center, Renmin Hospital of Wuhan University, Wuhan 430060, China.
Zhonghua Yu Fang Yi Xue Za Zhi. 2017 Mar 6;51(3):197-202. doi: 10.3760/cma.j.issn.0253-9624.2017.03.002.
To evaluate the effects of exposure to ozone (O(3)) on sperm quality during different stages of spermatogenesis. All 1 780 subjects attending to the Reproductive Medicine Center in Renmin Hospital of Wuhan University were recruited from April, 4, 2013 to June, 30, 2015. The subjects were living in Wuhan more than 3 months before attending to the program, aged 20 to 40 years. Semen quality (sperm concentration and sperm count) were measured according to standardized protocols. Corresponding daily 8 hours average concentration of O(3), other polluted concentration, average temperature and relative humidity were collected in different time, including lag 0, 10, 70 and 90 d, and lag 0-9 d, lag 10-14 d, lag 70-90 d and lag 0-90 d. After controlling the age, BMI, education level and other confounders, generalized linear Model was used to investigate the association between O(3) and sperm quality during different stages of spermatogenesis. Average daily concentration of O(3) during the study period was (114.20±74.88) μg/m(3) and the mean values of sperm concentration and count were (76.32±50.17) millions/ml and (164.77 ± 133.05) millions/sample, respectively. Exposure to O(3) was associated with decreasing sperm concentration and count. For every 1 μg/m(3) increase of O(3), the decrease of sperm concentration during lag 10, lag 0-9 and lag 10-14 days exposure windows were 0.040 (95% 0.004-0.077) millions/ml, 0.081 (95% 0.003-0.158) millions/ml and 0.059 (95% 0.001-0.116) millions/ml, respectively. And the decrease of sperm count during lag 10, lag 0-9 days exposure windows were 0.105 (95% 0.008-0.202) millions/sample and 0.221 (95% 0.016-0.426) millions/sample. After stratification, in the ozone concentration (50) and ≥(50) groups, and the number of subjects in each exposure windows (lag 0-9, lag 10-14, lag 70-90, lag 0-90 days) were 887 and 893, 890 and 890, 895 and 885, 889 and 891, respectively. Compared with the high ozone concentration group, the effects of low group were more obvious. Furthermore, the effects of low concentration group was the most obvious during lag 10-14 days, for every 1 μg/m(3) increase of O(3), the decrease of sperm concentration was 0.249 (95% 0.028-0.470) millions/ml. After sensitivity analysis, the effects of exposure to ozone on sperm concentration and sperm count remained relatively unchanged. Our study suggested that exposure to O(3) was significantly associated with decreasing semen quality in Wuhan. Moreover, the effects were more obvious during lag 0-9 and lag 10-14 days.
评估臭氧（O₃）暴露在精子发生不同阶段对精子质量的影响。2013年4月4日至2015年6月30日，招募了武汉大学人民医院生殖医学中心的1780名受试者。受试者在参加该项目前已在武汉居住超过3个月，年龄在20至40岁之间。根据标准化方案测量精液质量（精子浓度和精子计数）。收集不同时间（包括滞后0、10、70和90天，以及滞后0 - 9天、滞后10 - 14天、滞后70 - 90天和滞后0 - 90天）的每日8小时平均O₃浓度、其他污染浓度、平均温度和相对湿度。在控制年龄、体重指数、教育水平和其他混杂因素后，使用广义线性模型研究O₃与精子发生不同阶段精子质量之间的关联。研究期间O₃的日均浓度为（114.20±74.88）μg/m³，精子浓度和计数的平均值分别为（76.32±50.17）百万/ml和（164.77±133.05）百万/样本。O₃暴露与精子浓度和计数的降低有关。O₃每增加1μg/m³，在滞后10天、滞后0 - 9天和滞后10 - 14天暴露窗口期间精子浓度的降低分别为0.040（95% 0.004 - 0.077）百万/ml、0.081（95% 0.003 - 0.158）百万/ml和0.059（95% 0.001 - 0.116）百万/ml。在滞后10天、滞后0 - 9天暴露窗口期间精子计数的降低分别为0.105（95% 0.008 - 0.202）百万/样本和0.221（95% 0.016 - 0.426）百万/样本。分层后，在臭氧浓度＜50和≥50组中，每个暴露窗口（滞后0 - 9天、滞后10 - 14天、滞后70 - 90天、滞后0 - 90天）的受试者数量分别为887和893、890和890、895和885、889和891。与高臭氧浓度组相比，低浓度组的影响更明显。此外，低浓度组在滞后10 - 14天的影响最明显，O₃每增加1μg/m³，精子浓度降低0.249（95% 0.028 - 0.470）百万/ml。敏感性分析后，臭氧暴露对精子浓度和精子计数的影响保持相对不变。我们的研究表明，在武汉，O₃暴露与精液质量下降显著相关。此外，在滞后0 - 9天和滞后10 - 14天影响更明显。
