Sussman R, Resnick J, Calame K, Baluch J
Proc Natl Acad Sci U S A. 1978 Dec;75(12):5817-21. doi: 10.1073/pnas.75.12.5817.
In direct binding assays, purified lambdaind+ repressor displayed high affinity for nonoperator DNA containing single-strand gaps. Its affinity for this same DNA but completely double-stranded, nicked, or denatured was considerably lower. In contrast, purified lambdaind- repressor had 1/10th the affinity for the gapped DNA, a level comparable to that of purified lac repressor. In the presence of limiting amounts of ind+ repressor, nonoperator DNA containing gaps could be shown to compete effectively with lambda DNA for binding of repressor. A previous model of lambda induction [Sussman, R. & Ben-Zeev, H. (1975) Proc. Natl. Acad. Sci. USA 72, 1973--1976], based on the assumption that this phenomenon involves the binding of repressor to lesions in the host DNA, is reevaluated in the light of the data reported here.
在直接结合试验中，纯化的λind⁺阻遏物对含有单链缺口的非操纵子DNA表现出高亲和力。它对同样的DNA但完全双链、有切口或变性的情况的亲和力则低得多。相比之下，纯化的λind⁻阻遏物对有缺口的DNA的亲和力是前者的1/10，这一水平与纯化的乳糖阻遏物相当。在存在限量的ind⁺阻遏物的情况下，含有缺口的非操纵子DNA能有效地与λDNA竞争阻遏物的结合。基于这种现象涉及阻遏物与宿主DNA损伤结合这一假设的λ诱导的先前模型[Sussman, R. & Ben-Zeev, H. (1975) Proc. Natl. Acad. Sci. USA 72, 1973--1976]，根据此处报告的数据重新进行了评估。
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