Ruiz i Altaba A, Perry-O'Keefe H, Melton D A
Department of Biochemistry and Molecular Biology, Harvard University, Cambridge, MA 02138.
EMBO J. 1987 Oct;6(10):3065-70. doi: 10.1002/j.1460-2075.1987.tb02613.x.
The Xenopus laevis genome was screened for putative DNA-binding gene products by using the 'finger' region of the Drosophila gene Krüppel as a probe. The one gene detected, named Xfin, codes for a protein with 37 finger domains that comprise nearly 90% of the protein. In the light of studies by Rhodes and Klug (Cell, 46, 123-132, 1986), these data suggest that the Xfin protein has the capacity to bind an unusually large stretch (185 bases) of DNA. The Xfin gene is expressed as a maternal and zygotic mRNA that undergoes extensive polyadenylation changes during early development. The Xfin mRNA expression pattern and the potential DNA binding activity of the protein point to the possibility that the Xfin gene may have a role in controlling gene activity during early embryonic development.
通过使用果蝇基因Krüppel的“指状”区域作为探针，对非洲爪蟾的基因组进行筛选，以寻找假定的DNA结合基因产物。检测到的一个名为Xfin的基因编码一种含有37个指状结构域的蛋白质，这些结构域占该蛋白质的近90%。根据罗德兹和克鲁格的研究（《细胞》，第46卷，第123 - 132页，1986年），这些数据表明Xfin蛋白有能力结合一段异常长（185个碱基）的DNA。Xfin基因以母源和合子mRNA的形式表达，在早期发育过程中经历广泛的多聚腺苷酸化变化。Xfin mRNA的表达模式以及该蛋白质潜在的DNA结合活性表明，Xfin基因可能在早期胚胎发育过程中控制基因活性方面发挥作用。
