从海洋来源真菌 Fusidioides 枝顶孢菌 RZ01 中分离鉴定两种新化合物。

Isolation and identification of two new compounds from marine-derived fungus Acremonium fusidioides RZ01.

作者信息

An Xiao, Feng Bao-Min, Chen Gang, Chen Shao-Fei, Wang Hai-Feng, Pei Yue-Hu

机构信息

Key Laboratory of Structure-Based Drug Design & Discovery, Ministry of Education, Shenyang Pharmaceutical University, Shenyang 110016, China; School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China.

School of Life Sciences and Biotechnology, Dalian University, Dalian 116622, China.

出版信息

Chin J Nat Med. 2016 Dec;14(12):934-938. doi: 10.1016/S1875-5364(17)30019-5.

DOI:10.1016/S1875-5364(17)30019-5

PMID:28262121

Abstract

Two new compounds, (22E)-25-carboxy-8β,14β-epoxy-4α,5α-dihydroxyergosta-2,22-dien-7-one (1) and fusidione (3), along with two known compounds, 5α,8α-epidioxy ergosta-6,22-diene-3β-ol (2) and microperfuranone (4), were isolated from the fermentation products of the marine-sourced fungus Acremonium fusidioides RZ01. The structures of compounds 1 and 3 were elucidated by extensive spectroscopic methods, especially 2D NMR, and their absolute configurations were suggested on the basis of the circular dichroism spectral analysis and the NOESY data. Both new compounds showed inhibitory activity against HL-60 cells with IC values being16.6 and 44.9 μmol·L, respectively.

摘要

从海洋来源的镰刀菌Acremonium fusidioides RZ01的发酵产物中分离出两种新化合物，即(22E)-25-羧基-8β,14β-环氧-4α,5α-二羟基麦角甾-2,22-二烯-7-酮（1）和夫西地酮（3），以及两种已知化合物，5α,8α-环氧麦角甾-6,22-二烯-3β-醇（2）和微呋喃酮（4）。化合物1和3的结构通过广泛的光谱方法，特别是二维核磁共振（2D NMR）得以阐明，并且基于圆二色光谱分析和核Overhauser效应光谱（NOESY）数据确定了它们的绝对构型。两种新化合物均对HL-60细胞显示出抑制活性，其半数抑制浓度（IC）值分别为16.6和44.9 μmol·L。