Swierenga S H, MacManus J P, Whitfield J F
In Vitro. 1976 Jan;12(1):31-6. doi: 10.1007/BF02832790.
Cells of primary and secondary cultures of rat heart ventricle were grown in a medium supplemented with homologous plasma to avoid the non-specific proliferogenic effects of foreign sera. Specific chelation of calcium from the medium arrested the cells' progression through their cycle at the G1 phase. A permanent or brief restoration of the extracellular calcium level (48 hr later) was followed, after a 5 to 6 hr delay, by a burst of DNA synthesis, and the resumption of mitotic activity.
大鼠心室原代和传代培养细胞在补充了同源血浆的培养基中生长，以避免外源血清的非特异性增殖效应。从培养基中特异性螯合钙会使细胞周期在G1期停滞。细胞外钙水平永久性或短暂恢复（48小时后），经过5至6小时的延迟后，会伴随DNA合成爆发以及有丝分裂活性的恢复。
