Malekos Eric, Montano Christy, Carpenter Susan
Department of Biomolecular Engineering, University of California Santa Cruz, Santa Cruz, CA, USA.
Department of Molecular, Cell, and Developmental Biology, University of California Santa Cruz, Santa Cruz, CA, USA.
BMC Genomics. 2025 Jul 1;26(1):607. doi: 10.1186/s12864-025-11775-8.
We present CRISPRware, an efficient method for generating guide RNA (gRNA) libraries against transcribed, translated, and noncoding regions. CRISPRware leverages next-generation sequencing data to design context-specific gRNAs and can account for genetic variation, which allows allele-specific guide design on a genome-wide scale. As a demonstration of use and to create a publicly available resource, we use CRISPRware to identify and score gRNAs against coding sequences in six model organisms for Cas9 and Cas12A and host these in a publicly available session on the UCSC Genome Browser.
我们展示了CRISPRware，这是一种针对转录区域、翻译区域和非编码区域生成向导RNA（gRNA）文库的有效方法。CRISPRware利用下一代测序数据来设计特定背景的gRNA，并能够考虑到遗传变异，从而实现全基因组范围内的等位基因特异性向导设计。作为应用示范并创建一个公开可用的资源，我们使用CRISPRware针对六种模式生物的编码序列识别gRNA并进行评分，用于Cas9和Cas12A，并将这些gRNA托管在加州大学圣克鲁兹分校基因组浏览器的一个公开会话中。
