大肠杆菌attTn7的序列要求，转座子Tn7插入的一个特定位点。
Sequence requirements of Escherichia coli attTn7, a specific site of transposon Tn7 insertion.
作者信息
McKown R L, Orle K A, Chen T, Craig N L
机构信息
Department of Microbiology and Immunology, University of California, San Francisco 94143.
出版信息
J Bacteriol. 1988 Jan;170(1):352-8. doi: 10.1128/jb.170.1.352-358.1988.
Abstract
Transposon Tn7 transposes at high frequency to a specific site, attTn7, in the Escherichia coli chromosome. We devised a quantitative assay for Tn7 transposition in which Tn7-end derivatives containing the cis-acting transposition sequences of Tn7 transpose from a bacteriophage lambda vector upon infection into cells containing the Tn7-encoded transposition proteins. We used this assay to identify a 68-base-pair DNA segment containing the sequences essential for attTn7 target activity. This segment is positioned asymmetrically with respect to the specific point of Tn7 insertion in attTn7 and lacks obvious homology to the sequences at the ends of Tn7 which participate directly in transposition. We also show that some sequences essential for attTn7 target activity are contained within the protein-coding sequence of a bacterial gene.
摘要
转座子Tn7以高频转座至大肠杆菌染色体中的一个特定位点attTn7。我们设计了一种用于Tn7转座的定量测定法，其中包含Tn7顺式作用转座序列的Tn7末端衍生物在感染含有Tn7编码转座蛋白的细胞后，从噬菌体λ载体中转座。我们使用该测定法鉴定出一个68个碱基对的DNA片段，其包含attTn7靶标活性所必需的序列。该片段相对于attTn7中Tn7插入的特定位点不对称定位，并且与直接参与转座的Tn7末端序列缺乏明显的同源性。我们还表明，attTn7靶标活性所必需的一些序列包含在一个细菌基因的蛋白质编码序列内。
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