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水痘-带状疱疹病毒抗体的五种检测方法与膜抗原荧光抗体检测法的比较

Comparison of five assays for antibody to varicella-zoster virus and the fluorescent-antibody-to-membrane-antigen test.

作者信息

Larussa P, Steinberg S, Waithe E, Hanna B, Holzman R

机构信息

Department of Pediatrics, College of Physicians & Surgeons, Columbia University, New York, New York 10032.

出版信息

J Clin Microbiol. 1987 Nov;25(11):2059-62. doi: 10.1128/jcm.25.11.2059-2062.1987.

Abstract

Three commercially available assays (the Varicelisa Test Kit [Whittaker M.A. Bioproducts, Walkersville, Md.], the VZV Indirect Fluorescent-Antibody Test [Electro-Nucleonics, Inc., Columbia, Md.], and the Litton VZV Bio-EnzaBead Screen Kit [Litton Bionetics, Inc., Charleston, S.C.]) and two enzyme-linked immunosorbent assays used in our laboratory, one using a membrane-associated antigen and the other using a soluble antigen dotted on nitrocellulose paper, were compared with a varicella-zoster virus antibody reference assay, the fluorescent-antibody-to-membrane-antigen test. All of the assays compared favorably to the fluorescent-antibody-to-membrane-antigen test when evaluated for sensitivity (0.95), specificity (0.84), and test-retest reliability (79 to 96%), except for the Litton assay, which demonstrated significantly different results for all of the parameters tested (0.55, 1.0, and 69%, respectively).

摘要

三种市售检测方法(水痘酶联免疫吸附试验试剂盒[惠特克医学产品公司,马里兰州沃克维尔]、水痘-带状疱疹病毒间接荧光抗体检测法[电子核子学公司,马里兰州哥伦比亚]以及利顿水痘-带状疱疹病毒生物酶标筛选试剂盒[利顿生物遗传学公司,南卡罗来纳州查尔斯顿])和我们实验室使用的两种酶联免疫吸附试验,一种使用膜相关抗原,另一种使用点样于硝酸纤维素纸上的可溶性抗原,与水痘-带状疱疹病毒抗体参考检测方法即荧光抗体-膜抗原检测法进行了比较。在评估敏感性(0.95)、特异性(0.84)和重测可靠性(79%至96%)时,除利顿检测法外,所有检测方法与荧光抗体-膜抗原检测法相比均表现良好,利顿检测法在所有测试参数上显示出显著不同的结果(分别为0.55、1.0和69%)。

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