Larussa P, Steinberg S, Waithe E, Hanna B, Holzman R
Department of Pediatrics, College of Physicians & Surgeons, Columbia University, New York, New York 10032.
J Clin Microbiol. 1987 Nov;25(11):2059-62. doi: 10.1128/jcm.25.11.2059-2062.1987.
Three commercially available assays (the Varicelisa Test Kit [Whittaker M.A. Bioproducts, Walkersville, Md.], the VZV Indirect Fluorescent-Antibody Test [Electro-Nucleonics, Inc., Columbia, Md.], and the Litton VZV Bio-EnzaBead Screen Kit [Litton Bionetics, Inc., Charleston, S.C.]) and two enzyme-linked immunosorbent assays used in our laboratory, one using a membrane-associated antigen and the other using a soluble antigen dotted on nitrocellulose paper, were compared with a varicella-zoster virus antibody reference assay, the fluorescent-antibody-to-membrane-antigen test. All of the assays compared favorably to the fluorescent-antibody-to-membrane-antigen test when evaluated for sensitivity (0.95), specificity (0.84), and test-retest reliability (79 to 96%), except for the Litton assay, which demonstrated significantly different results for all of the parameters tested (0.55, 1.0, and 69%, respectively).
三种市售检测方法（水痘酶联免疫吸附试验试剂盒[惠特克医学产品公司，马里兰州沃克维尔]、水痘-带状疱疹病毒间接荧光抗体检测法[电子核子学公司，马里兰州哥伦比亚]以及利顿水痘-带状疱疹病毒生物酶标筛选试剂盒[利顿生物遗传学公司，南卡罗来纳州查尔斯顿]）和我们实验室使用的两种酶联免疫吸附试验，一种使用膜相关抗原，另一种使用点样于硝酸纤维素纸上的可溶性抗原，与水痘-带状疱疹病毒抗体参考检测方法即荧光抗体-膜抗原检测法进行了比较。在评估敏感性（0.95）、特异性（0.84）和重测可靠性（79%至96%）时，除利顿检测法外，所有检测方法与荧光抗体-膜抗原检测法相比均表现良好，利顿检测法在所有测试参数上显示出显著不同的结果（分别为0.55、1.0和69%）。
