D亚属人类腺病毒：9型和19型的限制性图谱分析及一种新基因组类型的特征描述

Human adenovirus from subgenus D: restriction mapping of types 9 and 19 and characterization of a new genome type.

作者信息

Gomes S A, Niel C

机构信息

Department of Virology, Oswaldo Cruz Foundation, Rio de Janiero, Brazil.

出版信息

J Gen Virol. 1987 Dec;68 ( Pt 12):3171-5. doi: 10.1099/0022-1317-68-12-3171.

DOI:10.1099/0022-1317-68-12-3171

PMID:2826655

Abstract

A strain of adenovirus (AV) was isolated from the stools of a child with acute gastroenteritis. This virus (strain 208) was specifically neutralized by antiserum to AV19, a species belonging to subgenus D and previously reported to cause keratoconjunctivitis. However, restriction analysis of strain 208 showed it to be distinct from AV19 and the other 40 human AV serotypes presently known. When calculated from 18 restriction patterns, the proportion of comigrating fragments common to AV19 and strain 208 was only 57%. This percentage was no higher than that obtained by comparison of strain 208 and AV9, a randomly chosen type from subgenus D. Strain 208 therefore appeared to represent a new genome type. The restriction maps of the three viruses for BamHI, ClaI, EcoRI, HindIII, MluI, NdeI and SfiI endonucleases are presented.

摘要

从一名患有急性肠胃炎儿童的粪便中分离出一株腺病毒（AV）。该病毒（208株）被抗AV19血清特异性中和，AV19属于D亚属，此前报道可引起角膜结膜炎。然而，对208株病毒的限制性分析表明，它与AV19以及目前已知的其他40种人类AV血清型不同。根据18种限制性图谱计算，AV19和208株共迁移片段的比例仅为57%。该百分比并不高于208株与从D亚属中随机选择的AV9型比较所得到的比例。因此，208株病毒似乎代表一种新的基因组类型。文中给出了这三种病毒针对BamHI、ClaI、EcoRI、HindIII、MluI、NdeI和SfiI核酸内切酶的限制性图谱。