Restriction maps of human adenovirus types 2, 5, and 3 for BstEII, MluI, NdeI, NruI and SfiI endonucleases.

The positions of cleavage sites for BstEII, MluI, NdeI, NruI and SfiI restriction endonucleases in the DNA from human adenovirus (Ad) serotypes 2, 5 and 3 were determined. In addition, the sites of cleavage for BglII in Ad3 DNA were located. All these enzymes possess a narrow specificity and generated a small number of discrete DNA fragments. Ad3 DNA was not cleaved by MluI and SfiI. It was the first observation of the absence of cleavage of an adenovirus DNA by a restriction endonuclease.