School of Food & Biological Engineering, Jiangsu University, Zhenjiang 212013, China; Physical Processing of Agricultural Products Key Lab of Jiangsu Province, Zhenjiang 212013, Jiangsu, China.
School of Food & Biological Engineering, Jiangsu University, Zhenjiang 212013, China.
Ultrason Sonochem. 2018 Jan;40(Pt A):128-134. doi: 10.1016/j.ultsonch.2017.07.007. Epub 2017 Jul 6.
Ultrasound and three-phase partitioning (USTPP) were combined to extract and separate polysaccharides (PS) efficiently from Corbicula fluminea crude extracts. The following optimum experimental conditions were established: 20% (w/v) ammonium sulfate concentration, 1:1 (v/v) t-butanol to crude extract ratio, 180W ultrasonic power, 40kHz frequency, 100% duty cycle, 10min irradiation time, and 35°C. The maximum extraction yield of PS obtained from USTPP was approximately 11.22%, which was higher than the maximum yields from conventional three-phase partitioning (TPP; 9.32%) and ultrasound extraction (USE; 6.05%). The extraction time for USTPP was significantly reduced to 10min from 30 and 60min for TPP and USE methods, respectively. The primary chemical structures of PS collected through the three extraction protocols were basically conserved. The synergistic effects of ultrasound and TPP on PS extraction were also determined. Results revealed that USTPP is an effective technique to extract and separate PS from C. fluminea potentially applied in food, cosmetics, and medicines.
超声辅助三相分配法（USTPP）被用于从河蚬粗提物中高效提取和分离多糖（PS）。以下是优化实验条件：20%（w/v）硫酸铵浓度，正丁醇与粗提取物体积比为 1:1，超声功率 180W，频率 40kHz，占空比 100%，辐照时间 10min，温度 35°C。与传统三相分配法（TPP；9.32%）和超声提取法（USE；6.05%）相比，USTPP 法从河蚬中提取 PS 的最大产率约为 11.22%。USTPP 的提取时间从 TPP 和 USE 方法的 30min 和 60min 分别显著缩短至 10min。通过三种提取方法得到的 PS 的主要化学结构基本保持一致。还确定了超声和 TPP 对 PS 提取的协同作用。结果表明，USTPP 是一种从河蚬中提取和分离 PS 的有效技术，有望应用于食品、化妆品和医药领域。
