Dall'Olio F, Malagolini N, Campadelli-Fiume G, Serafini-Cessi F
Dipartimento di Patologia Sperimentale dell' Università di Bologna, Italy.
Arch Virol. 1987;97(3-4):237-49. doi: 10.1007/BF01314424.
The changes in the apparent molecular weight of herpes simplex virus type 2 glycoprotein G (gG2) were studied by using different [3H] mannose labeling time intervals. Various size classes of precursors, probably derived from proteolytic cleavage of the translational product, were identified. Our experiments provide evidence that only the 74 Kd species is the real precursor of the mature 120 Kd gG2. The increase in size is due for the most part to the assembly of O-linked oligosaccharides and to a lesser extent to the conversion of N-linked chains to fucosylated diantennary species.
通过使用不同的[³H]甘露糖标记时间间隔，研究了单纯疱疹病毒2型糖蛋白G（gG2）表观分子量的变化。鉴定出了各种大小类别的前体，它们可能源自翻译产物的蛋白水解切割。我们的实验提供了证据，表明只有74 Kd的物种是成熟的120 Kd gG2的真正前体。大小的增加主要归因于O-连接寡糖的组装，在较小程度上归因于N-连接链向岩藻糖基化二天线物种的转化。
