Abe K, Konaka R
Shionogi Biomedical Laboratories, Shionogi & Co., Ltd., Osaka, Japan.
Clin Chem. 1988 Jan;34(1):87-90.
We describe a "high-performance" liquid-chromatographic method for determining 3-methoxy-4-hydroxyphenylethylene glycol (MHPG) in human urine. MHPG is separated on a reversed-phase column with isocratic elution, oxidized with sodium metaperiodate, and its absorbance measured at 365 nm. This method shows higher specificity, less interference for MHPG than methods involving electrochemical or fluorescence detection. Post-column derivatization of MHPG with periodate yields vanillin. The detection limit (twice the signal-to-noise ratio) in urine samples was 0.08 mg/L. Mean analytical recovery was 72%. Within-assay and day-to-day CVs were 2.9% and 6.5%, respectively. Reference intervals for MHPG in 24-h urine from apparently healthy subjects were 0.85-3.24 mg/day for men and 0.63-2.20 mg/day for women. In terms of creatinine excretion, the respective reference intervals were 0.55-1.99 and 0.70-1.96 mg per gram of creatinine.
我们描述了一种用于测定人尿中3-甲氧基-4-羟基苯乙二醇（MHPG）的“高效”液相色谱法。MHPG在反相柱上通过等度洗脱进行分离，用过碘酸钠氧化，然后在365nm处测量其吸光度。与涉及电化学或荧光检测的方法相比，该方法显示出更高的特异性，对MHPG的干扰更少。MHPG与高碘酸盐的柱后衍生反应产生香草醛。尿样中的检测限（信噪比的两倍）为0.08mg/L。平均分析回收率为72%。批内和日间变异系数分别为2.9%和6.5%。显然健康受试者24小时尿中MHPG的参考区间，男性为0.85 - 3.24mg/天，女性为0.63 - 2.20mg/天。以肌酐排泄量计，各自的参考区间为每克肌酐0.55 - 1.99mg和0.70 - 1.96mg。
