Shen Yue, Wang Yun, Chen Tai, Gao Feng, Gong Jianhui, Abramczyk Dariusz, Walker Roy, Zhao Hongcui, Chen Shihong, Liu Wei, Luo Yisha, Müller Carolin A, Paul-Dubois-Taine Adrien, Alver Bonnie, Stracquadanio Giovanni, Mitchell Leslie A, Luo Zhouqing, Fan Yanqun, Zhou Baojin, Wen Bo, Tan Fengji, Wang Yujia, Zi Jin, Xie Zexiong, Li Bingzhi, Yang Kun, Richardson Sarah M, Jiang Hui, French Christopher E, Nieduszynski Conrad A, Koszul Romain, Marston Adele L, Yuan Yingjin, Wang Jian, Bader Joel S, Dai Junbiao, Boeke Jef D, Xu Xun, Cai Yizhi, Yang Huanming
BGI-Shenzhen, Shenzhen 518083, China.
School of Biological Sciences, University of Edinburgh, Edinburgh EH9 3BF, UK.
Science. 2017 Mar 10;355(6329). doi: 10.1126/science.aaf4791.
Here, we report the successful design, construction, and characterization of a 770-kilobase synthetic yeast chromosome II (synII). Our study incorporates characterization at multiple levels-including phenomics, transcriptomics, proteomics, chromosome segregation, and replication analysis-to provide a thorough and comprehensive analysis of a synthetic chromosome. Our Trans-Omics analyses reveal a modest but potentially relevant pervasive up-regulation of translational machinery observed in synII, mainly caused by the deletion of 13 transfer RNAs. By both complementation assays and SCRaMbLE (synthetic chromosome rearrangement and modification by -mediated evolution), we targeted and debugged the origin of a growth defect at 37°C in glycerol medium, which is related to misregulation of the high-osmolarity glycerol response. Despite the subtle differences, the synII strain shows highly consistent biological processes comparable to the native strain.
在此，我们报告了一条770千碱基对的合成酵母二号染色体（synII）的成功设计、构建及表征。我们的研究纳入了多个层面的表征，包括表型组学、转录组学、蛋白质组学、染色体分离及复制分析，以对一条合成染色体进行全面且综合的分析。我们的跨组学分析揭示，在synII中观察到翻译机制有适度但可能相关的普遍上调，这主要是由13个转运RNA的缺失导致的。通过互补试验和SCRaMbLE（通过介导进化进行合成染色体重排和修饰），我们针对并调试了甘油培养基中37°C时生长缺陷的根源，该缺陷与高渗甘油应答的调控异常有关。尽管存在细微差异，但synII菌株显示出与天然菌株高度一致的生物学过程。
