无机纳米颗粒通过氧化还原状态和线粒体膜电位的变化来杀死细胞。

Inorganic nanoparticles kill via changes in redox status and mitochondrial membrane potential.

作者信息

Adeyemi Oluyomi Stephen, Murata Yuho, Sugi Tatsuki, Kato Kentaro

机构信息

National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Japan; Medicinal Biochemistry and Toxicology Laboratory, Department of Biological Sciences, Landmark University, Omu-Aran, Nigeria.

National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Japan.

出版信息

Int J Nanomedicine. 2017 Feb 28;12:1647-1661. doi: 10.2147/IJN.S122178. eCollection 2017.

This study evaluated the anti- potential of gold, silver, and platinum nanoparticles (NPs). Inorganic NPs (0.01-1,000 µg/mL) were screened for antiparasitic activity. The NPs caused >90% inhibition of growth with EC values of ≤7, ≤1, and ≤100 µg/mL for gold, silver, and platinum NPs, respectively. The NPs showed no host cell cytotoxicity at the effective anti- concentrations; the estimated selectivity index revealed a ≥20-fold activity toward the parasite versus the host cell. The anti- activity of the NPs, which may be linked to redox signaling, affected the parasite mitochondrial membrane potential and parasite invasion, replication, recovery, and infectivity potential. Our results demonstrated the antiparasitic potential of NPs. The findings support the further exploration of NPs as a possible source of alternative and effective anti- agents.

本研究评估了金、银和铂纳米颗粒（NPs）的抗寄生虫潜力。对无机纳米颗粒（0.01 - 1000 µg/mL）进行了抗寄生虫活性筛选。这些纳米颗粒分别对金、银和铂纳米颗粒产生了>90%的生长抑制，其半数效应浓度（EC）值分别≤7、≤1和≤100 µg/mL。在有效的抗寄生虫浓度下，这些纳米颗粒对宿主细胞无细胞毒性；估计的选择性指数显示，纳米颗粒对寄生虫的活性比对宿主细胞高≥20倍。纳米颗粒的抗寄生虫活性可能与氧化还原信号传导有关，影响了寄生虫的线粒体膜电位以及寄生虫的侵袭、复制、恢复和感染潜力。我们的结果证明了纳米颗粒的抗寄生虫潜力。这些发现支持进一步探索纳米颗粒作为替代和有效抗寄生虫药物的可能来源。