Sheu C W, Moreland F M, Lee J K, Dunkel V C
Genetic Toxicology Branch, Food and Drug Administration, Washington, DC 20204.
Environ Mol Mutagen. 1988;11(1):41-8. doi: 10.1002/em.2850110106.
The spermicidal surfactant nonoxynol-9 (Igepal CO-630, GAF Corp.) and a potential impurity, 1,4-dioxane, were tested in the in vitro cell transformation assay using BALB/3T3 cells. Two treatment periods, 48 hr and 13 days, were used. Nonoxynol-9, tested at levels up to 10 micrograms/ml, did not induce transformation, whereas dioxane was very active in the induction of type III foci in the cultured BALB/3T3 cells.
使用BALB/3T3细胞，在体外细胞转化试验中对杀精表面活性剂壬苯醇醚-9（Igepal CO-630，GAF公司）及其潜在杂质1,4-二恶烷进行了测试。采用了两个处理期，即48小时和13天。浓度高达10微克/毫升的壬苯醇醚-9未诱导转化，而二恶烷在培养的BALB/3T3细胞中诱导III型病灶方面非常活跃。
