Gadd A L, Curtis-Prior P B
Napp Research Centre, Cambridge Science Park, UK.
J Pharm Pharmacol. 1988 Mar;40(3):215-6. doi: 10.1111/j.2042-7158.1988.tb05225.x.
The transmembrane migration technique, a simple method in-vitro for quantitatively assessing the effects of a drug on human sperm motility, has been evaluated. The original method has been modified to include a preincubation step, and the incubation time has been reduced to 90 min. In semen samples possessing sperm concentrations of less than 75 x 12(6) spermatozoa mL-1 the volume of the lower reservoir has been reduced to 1 mL. This modified method has been used to compare the spermicidal potency of the widely employed non-ionic surfactant nonoxynol-9, with nonoxynol-5 and nonoxynol-15 (containing, respectively, fewer and more ethylene oxide units per molecule). The rank order of spermicidal potency of the compounds evaluated was nonoxynol-9 = nonoxynol-5 greater than nonoxynol-15.
跨膜迁移技术是一种体外定量评估药物对人类精子活力影响的简单方法，已得到评估。原始方法已进行修改，增加了预孵育步骤，孵育时间缩短至90分钟。对于精子浓度低于75×10⁶/mL的精液样本，下部储液器的体积已减少至1 mL。这种改进后的方法已用于比较广泛使用的非离子表面活性剂壬苯醇醚-9与壬苯醇醚-5和壬苯醇醚-15（分别每分子含有较少和较多环氧乙烷单元）的杀精效力。所评估化合物的杀精效力排序为壬苯醇醚-9 = 壬苯醇醚-5大于壬苯醇醚-15。
