Institute of Marine Microbiology, College of Life Science, Dalian Minzu University, Dalian, 116600, Liaoning, People's Republic of China.
Curr Microbiol. 2020 Nov;77(11):3643-3650. doi: 10.1007/s00284-020-02181-2. Epub 2020 Sep 7.
Streptomyces sp. S501, which was isolated from the sediment of Yalujiang Estuary in China, was the first marine Streptomyces species discovered to act as an excellent petroleum degrader. We analyzed the effect of pH, temperature, and concentration of NHNO on the petroleum degradation of strain S501, and the optimum biodegradation rate reached 63.02% under the condition of 2 g/L NHNO addition at 30 °C and pH 8. The complete genome sequence of Streptomyces sp. S501 was determined by using the PacBio RSII platform, which contains a linear chromosome with 7,173,651 bp and a linear plasmid with 288,181 bp, with GC contents of 71.19% and 67.57%, respectively. The genome sequence suggests that Streptomyces sp. S501 has the ability to degrade several hazardous pollutants, as well as the ability to biosynthesize diverse secondary metabolites and enzymes. There are fifty annotated genes involved in oil component degradation, and there are three genes without known annotation information in Streptomyces sp. S501, which have high homology with genes encoding P450 family enzymes and should be novel genes involved in alkane degradation. This study provides useful genetic information for investigating the molecular mechanisms of marine Streptomyces, with biodegradation and application potential.
从中国鸭绿江口的沉积物中分离到的链霉菌 S501 是第一个被发现具有优异石油降解能力的海洋链霉菌。我们分析了 pH 值、温度和 NHNO 浓度对 S501 菌株石油降解的影响，在 30°C 和 pH8 条件下添加 2g/L NHNO 时，最佳生物降解率达到 63.02%。采用 PacBio RSII 平台测定了链霉菌 S501 的全基因组序列，该基因组由一条 7173651bp 的线性染色体和一条 288181bp 的线性质粒组成，GC 含量分别为 71.19%和 67.57%。基因组序列表明，链霉菌 S501 具有降解多种危险污染物的能力，以及生物合成多种次生代谢物和酶的能力。有五十个注释基因参与油成分降解，而 S501 中有三个基因没有已知的注释信息，与编码 P450 家族酶的基因具有高度同源性，应该是参与烷烃降解的新基因。本研究为研究海洋链霉菌的分子机制、生物降解和应用潜力提供了有用的遗传信息。
