Department of Biotechnology, Faculty of Natural Sciences and Technology, NTNU Norwegian University of Science and Technology, Sem Saelands v. 6/8, 7491, Trondheim, Norway.
División de Ciencias Ambientales, Instituto Potosino de Investigación Científica y Tecnológica (IPICyT), Camino a la Presa San José 2055, Col. Lomas 4a Seccion, 78216, San Luis Potosi, SLP, Mexico.
Appl Microbiol Biotechnol. 2017 Jun;101(12):5149-5162. doi: 10.1007/s00253-017-8160-5. Epub 2017 Mar 9.
The anammox activity of a freshwater anammox consortium was strongly inhibited at low-salinity level. Stepwise adaptation from 0 to 3 g NaCl L took 153 days. Further adaptation to high-salinity concentration (from 3 to 30 g L) took only 40 days, and no inhibition was observed. A comprehensive insight into the salinity-induced successions of the total and the anammox communities was obtained by 454 pyrosequencing of 16S rRNA gene amplicons and statistical analysis. A major succession in the anammox community was observed at 3 g L where the dominating population shifted from Candidatus Brocadia fulgida to Ca. Kuenenia stuttgartiensis. The latter dominated at high salinity and seemed to be essential for the high (˃96%) ammonium and nitrite removal efficiencies achieved. SIMPER analysis indicated that these two dominating anammox species explained most to the differences in community structure among samples and helped in identifying other important members at different salinities.
淡水厌氧氨氧化菌（anammox）菌剂的厌氧氨氧化活性在低盐度水平下受到强烈抑制。从 0 逐渐适应到 3g/L NaCl 需要 153 天。进一步适应高盐浓度（从 3 到 30g/L）仅需 40 天，且未观察到抑制现象。通过对 16S rRNA 基因扩增子进行 454 焦磷酸测序和统计分析，深入了解了盐度诱导的总菌和厌氧氨氧化菌群落的演替。在 3g/L 时观察到厌氧氨氧化菌群落的主要演替，优势种群从布鲁卡氏菌（Candidatus Brocadia fulgida）转变为斯图特氏菌（Ca. Kuenenia stuttgartiensis）。后者在高盐度下占主导地位，似乎对于实现高（˃96%）氨氮和亚硝酸盐去除效率至关重要。SIMPER 分析表明，这两种占主导地位的厌氧氨氧化菌对样品间群落结构的差异解释最多，并有助于确定不同盐度下的其他重要成员。
