Maruška Audrius, Ugenskienė Rasa, Raulinaitytė Danguolė, Juozaitytė Elona, Kaškonienė Vilma, Drevinskas Tomas, Stelmakienė Ada, Akuneca Ieva, Makaravičius Tomas, Tiso Nicola, Bartkuvienė Violeta, Kornyšova Olga, Ragažinskienė Ona, Ramanauskienė Kristina, Briedis Vitalis
Department of Biology, Faculty of Natural Sciences, Vytautas Magnus University, Kaunas, Lithuania.
Oncology Research Laboratory, Oncology Institute, Lithuanian University of Health Sciences, Kaunas, Lithuania.
Adv Med Sci. 2017 Mar;62(1):158-164. doi: 10.1016/j.advms.2016.08.002. Epub 2017 Mar 7.
To evaluate the antiproliferative effect of the aerial part of Chamerion angustifolium (L.) Holub. (Onagraceae) extract and its fractions in vitro. This is the first study on the anti-proliferative effect of C. angustifolium on 3 distinct breast cancer cell lines.
MATERIAL/METHODS: Breast cancer cell lines MCF7, MDA-MB-468 and MDA-MB-231 were exposed to different concentrations of the water extract of C. angustifolium, where DPPH radical scavenging activity was 0.018-0.443mg/ml, expressed in rutin equivalents. Cell growth was analyzed after 24, 48 and 72h of incubation. Solid-phase extraction was applied for the fractionation of C. angustifolium water extract and MDA-MB-468 cell line growth was tested using different fractions.
The concentrations corresponding to radical scavenging activity of 0.117 and 0.266mg/ml caused MCF7 cells growth inhibition, while in the samples exposed to the highest concentration (0.355 and 0.443mg/ml) no proliferation was register, suggesting cell death. MDA-MB-468 cell analysis showed similar responses. MDA-MB-231 demonstrated cell growth inhibition following the exposure to all analyzed high extract doses (0.117-0.443mg/ml). MDA-MB-468 cells were selected to evaluate the effect of fractions. In the samples exposed to the fraction containing the highest amount (91%) of oenothein B, at the concentration of 0.117mg/ml a pronounced cell growth inhibition while at higher concentrations (0.266 and 0.443mg/ml) no cell proliferation was observed.
The consumption of C. angustifolium herb can be advantageous, alongside with conventional breast cancer treatment.
评估柳叶菜（柳叶菜科）地上部分提取物及其馏分的体外抗增殖作用。这是关于柳叶菜对3种不同乳腺癌细胞系抗增殖作用的首次研究。
材料/方法：将乳腺癌细胞系MCF7、MDA-MB-468和MDA-MB-231暴露于不同浓度的柳叶菜水提取物中，其清除DPPH自由基活性为0.018 - 0.443mg/ml，以芦丁当量表示。孵育24、48和72小时后分析细胞生长情况。采用固相萃取法对柳叶菜水提取物进行分馏，并使用不同馏分测试MDA-MB-468细胞系的生长情况。
清除自由基活性对应浓度为0.117和0.266mg/ml时，可抑制MCF7细胞生长，而在暴露于最高浓度（0.355和0.443mg/ml）的样品中未检测到细胞增殖，提示细胞死亡。MDA-MB-468细胞分析显示出类似反应。MDA-MB-231细胞在暴露于所有分析的高提取物剂量（0.117 - 0.443mg/ml）后均表现出细胞生长抑制。选择MDA-MB-468细胞评估馏分的作用。在暴露于含有最高含量（91%）月见草素B的馏分的样品中，浓度为0.117mg/ml时细胞生长受到明显抑制，而在较高浓度（0.266和0.443mg/ml）下未观察到细胞增殖。
食用柳叶菜草本植物与传统乳腺癌治疗方法并用可能有益。
