L.乙醇提取物和乙酸乙酯提取物的植物化学分析、抗氧化及抗菌潜力

Phytochemical Profiling, Antioxidant and Antimicrobial Potentials of Ethanol and Ethyl Acetate Extracts of L.

作者信息

Kozhantayeva Akmaral, Tursynova Nurgul, Kolpek Ainagul, Aibuldinov Yelaman, Tursynova Arailym, Mashan Togzhan, Mukazhanova Zhazira, Ibrayeva Manshuk, Zeinuldina Aizhan, Nurlybayeva Aisha, Iskakova Zhanar, Tashenov Yerbolat

机构信息

Research Institute of New Chemical Technologies, L.N. Gumilyov Eurasian National University, Satpayev Street 2, Astana 010000, Kazakhstan.

Department of Chemistry, Faculty of Natural Sciences, L.N. Gumilyov Eurasian National University, Satpayev Street 2, Astana 010000, Kazakhstan.

出版信息

Pharmaceuticals (Basel). 2024 Jul 27;17(8):996. doi: 10.3390/ph17080996.

DOI:10.3390/ph17080996

PMID:39204101

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11357188/

Abstract

The study investigates the phytochemical profile, antioxidant capacity, and antimicrobial activities of ethanol (ChL-EtOH) and ethyl acetate (ChL-EtOAc) extracts from L. (ChL) harvested in Kazakhstan. The ChL-EtOH extract exhibited higher total phenolic (267.48 ± 3.44 mg GAE/g DE) and flavonoid content (24.18 ± 1.06 mg QE/g DE) compared to ChL-EtOAc. HPLC-UV-ESI/MS identified key phenolic acids and flavonoids, including gallic acid, chlorogenic acid, and quercetin 3-glucoside. FT-IR analysis confirmed the presence of characteristic functional groups. Antioxidant assays revealed strong DPPH scavenging and FRAP activities, with ChL-EtOH showing superior results (IC = 21.31 ± 0.65 μg/mL and 18.13 ± 0.15 μg/mL, respectively). Additionally, ChL-EtOH displayed notable antimicrobial efficacy against Gram-positive and Gram-negative bacteria, as well as the fungal strain . These findings suggest that ethanol extraction is more efficient for isolating bioactive compounds from ChL, underscoring its potential for pharmaceutical and nutraceutical applications.

摘要

该研究调查了从哈萨克斯坦采集的光果甘草（ChL）的乙醇提取物（ChL-EtOH）和乙酸乙酯提取物（ChL-EtOAc）的植物化学特征、抗氧化能力和抗菌活性。与ChL-EtOAc相比，ChL-EtOH提取物表现出更高的总酚含量（267.48±3.44 mg GAE/g DE）和黄酮含量（24.18±1.06 mg QE/g DE）。HPLC-UV-ESI/MS鉴定出了关键的酚酸和黄酮类化合物，包括没食子酸、绿原酸和槲皮素3-葡萄糖苷。傅里叶变换红外光谱（FT-IR）分析证实了特征官能团的存在。抗氧化试验显示出较强的二苯基苦味酰基自由基（DPPH）清除能力和铁离子还原抗氧化能力（FRAP），ChL-EtOH的结果更优（IC分别为21.31±0.65 μg/mL和18.13±0.15 μg/mL）。此外，ChL-EtOH对革兰氏阳性菌、革兰氏阴性菌以及真菌菌株均表现出显著的抗菌效果。这些发现表明，乙醇提取法从光果甘草中分离生物活性化合物的效率更高，突出了其在制药和营养保健品应用方面的潜力。