Wang Chaogang, Wang Kunpeng, Ning Jingjing, Luo Qiulan, Yang Yi, Huang Danqiong, Li Hui
Shenzhen Key Laboratory of Marine Bioresource and Eco-Environmental Science, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen, China.
Guangdong Technology Research Center for Marine Algal Bioengineering, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen, China.
Front Bioeng Biotechnol. 2021 Oct 25;9:650178. doi: 10.3389/fbioe.2021.650178. eCollection 2021.
The microalgae attracts attention for its ability to accumulate astaxanthin up to its 4% dry weight under stress conditions, such as high light, salt stress, and nitrogen starvation. Previous researches indicated that the regulation of astaxanthin synthesis might happen at the transcriptional level. However, the transcription regulatory mechanism of astaxanthin synthesis is still unknown in . Lacking studies on transcription factors (TFs) further hindered from discovering this mechanism. Hence, the transcriptome analysis of under the high light-sodium acetate stress for 1.5 h was performed in this study, aiming to discover TFs and the regulation on astaxanthin synthesis. In total, 83,869 unigenes were obtained and annotated based on seven databases, including NR, NT, Kyoto Encyclopedia of Genes and Genomes Orthology, SwissProt, Pfam, Eukaryotic Orthologous Groups, and Gene Ontology. Moreover, 476 TFs belonging to 52 families were annotated by blasting against the PlantTFDB database. By comparing with the control group, 4,367 differentially expressed genes composing of 2,050 upregulated unigenes and 2,317 downregulated unigenes were identified. Most of them were involved in metabolic process, catalytic activity, single-organism process, single-organism cellular process, and single-organism metabolic process. Among them, 28 upregulated TFs and 41 downregulated TFs belonging to 27 TF families were found. The transcription analysis showed that TFs had different transcription modules responding to the high light and sodium acetate stress. Interestingly, six TFs belonging to MYB, MYB_related, NF-YC, Nin-like, and C3H families were found to be involved in the transcription regulation of 27 astaxanthin synthesis-related genes according to the regulatory network. Moreover, these TFs might affect astaxanthin synthesis by directly regulating , showing that was the hub gene in astaxanthin synthesis. The present study provided new insight into a global view of TFs and their correlations to astaxanthin synthesis in .
微藻因其在高光、盐胁迫和氮饥饿等胁迫条件下能够积累高达其干重4%的虾青素的能力而备受关注。先前的研究表明，虾青素合成的调控可能发生在转录水平。然而，虾青素合成的转录调控机制在[具体研究对象，原文未明确]中仍然未知。缺乏对转录因子（TFs）的研究进一步阻碍了对该机制的发现。因此，本研究对[具体研究对象，原文未明确]在高光-醋酸钠胁迫1.5小时下进行了转录组分析，旨在发现转录因子以及对虾青素合成的调控。总共获得了83,869个单基因，并基于包括NR、NT、京都基因与基因组百科全书直系同源、SwissProt、Pfam、真核直系同源组和基因本体论在内的七个数据库进行了注释。此外，通过与植物转录因子数据库进行比对，注释了属于52个家族的476个转录因子。与对照组相比，鉴定出了4367个差异表达基因，其中包括2050个上调单基因和2317个下调单基因。它们中的大多数参与代谢过程、催化活性、单细胞过程、单细胞细胞过程和单细胞代谢过程。其中，发现了属于27个转录因子家族的28个上调转录因子和41个下调转录因子。转录分析表明，转录因子具有不同的转录模块来响应高光和醋酸钠胁迫。有趣的是，根据调控网络发现，属于MYB、MYB相关、NF-YC、Nin样和C3H家族的六个转录因子参与了27个虾青素合成相关基因的转录调控。此外，这些转录因子可能通过直接调控[原文未明确基因名称]来影响虾青素合成，表明[原文未明确基因名称]是虾青素合成中的枢纽基因。本研究为[具体研究对象，原文未明确]中转录因子及其与虾青素合成的相关性提供了新的见解。
