School of Biotechnology, Institute of Agricultural Technology, Suranaree University of Technology, 111 University avenue, Muang district, Nakhon Ratchasima 30000, Thailand.
Department of Chemical and Biomolecular Engineering, Vidyasirimedhi Institute of Science and Technology (VISTEC), Wangchan District, Rayong 21210, Thailand.
Bioresour Technol. 2017 Jun;233:342-352. doi: 10.1016/j.biortech.2017.02.114. Epub 2017 Feb 28.
The fermentation of succinic acid from fresh cassava root using Actinobacillus succinogenes ATCC55618, and the recovery of the product using crystallization were investigated. Fresh cassava root is an ideal succinic acid feedstock due to its low price and high starch content. Saccharification was carried out using commercially available enzymes and diammonium phosphate was used as an inexpensive nitrogen source. Different fermentation modes were compared in terms of product yield and productivity. Results for fed-batch fermentations showed that a succinic acid titer of 151.44g/L, with yield and productivity of 1.51g/g and 3.22g/L/h could be obtained. Seeded batch cooling crystallization was investigated after pre-treatment using nanofiltration. A succinic acid crystal purity of 99.35% with a relative crystallinity of 96.77% was obtained from high seeding experiments. These results indicated that fresh cassava roots could be an economically alternative feedstock for a high quality succinic acid production.
采用 Actinobacillus succinogenes ATCC55618 从新鲜木薯根中发酵生产琥珀酸，并通过结晶回收产物。新鲜木薯根是一种理想的琥珀酸原料，因为其价格低廉且淀粉含量高。糖化采用市售酶进行，使用磷酸二铵作为廉价的氮源。从产物产率和生产率方面比较了不同的发酵方式。分批补料发酵的结果表明，可以获得 151.44g/L 的琥珀酸浓度，产率和生产率分别为 1.51g/g 和 3.22g/L/h。采用纳滤预处理后，对种子批冷却结晶进行了研究。高接种实验得到的琥珀酸晶体纯度为 99.35%，相对结晶度为 96.77%。这些结果表明，新鲜木薯根可以作为高质量琥珀酸生产的一种经济替代原料。
