Machida Takuji, Takano Yuho, Iizuka Kenji, Machida Maiko, Hirafuji Masahiko
Department of Pharmacological Sciences, School of Pharmaceutical Sciences, Health Sciences University of Hokkaido, Ishikari-Tobetsu, Hokkaido 061-0293, Japan.
Division of Pharmacotherapy, Hokkaido Pharmaceutical University School of Pharmacy, Sapporo, Hokkaido 006-8590, Japan.
J Pharmacol Sci. 2017 Mar;133(3):190-193. doi: 10.1016/j.jphs.2017.02.009. Epub 2017 Feb 22.
This study aimed to investigate the acute and chronic effect of methotrexate on the intestinal substance P metabolism after a single administration to rats. Methotrexate caused a significant increase in the number of substance P-containing cells in the ileal mucosa both at 24 and 96 h. Most of enterochromaffin cells expressing l-tryptophan hydroxylase contained substance P. The expression of Tac1 mRNA was increased by methotrexate at 24 h, but not at 96 h. Thus, methotrexate causes acute hyperplasia of enterochromaffin cells in the intestinal mucosa of rats with a transient increase in the production of substance P.
本研究旨在探讨甲氨蝶呤单次给药后对大鼠肠道P物质代谢的急性和慢性影响。甲氨蝶呤在24小时和96小时时均导致回肠黏膜中含P物质的细胞数量显著增加。大多数表达l - 色氨酸羟化酶的肠嗜铬细胞含有P物质。甲氨蝶呤在24小时时增加了Tac1 mRNA的表达，但在96小时时未增加。因此，甲氨蝶呤可导致大鼠肠黏膜中肠嗜铬细胞急性增生，并使P物质产生短暂增加。
