Tenser R B, Hyman R W
Yale J Biol Med. 1987 Mar-Apr;60(2):159-67.
Latent herpes simplex virus (HSV) infection of the trigeminal ganglion of guinea pigs and latent varicella-zoster virus (VZV) infection of the trigeminal ganglion of humans were studied by in situ nucleic acid hybridization. Guinea pig trigeminal ganglia were removed during the period of viral latency (four to five weeks after corneal inoculation of HSV), and human ganglia were removed at autopsy. Radiolabeled HSV and VZV DNAs were used to probe ganglion tissue sections for viral-specified RNA. Hybridization detected only over neurons was present in 46 percent of ganglia from 22 latently infected guinea pigs and from 33 percent of ganglia from 10 human subjects. These results support the conclusion that some viral transcription occurred during HSV and VZV latency.
通过原位核酸杂交技术研究了豚鼠三叉神经节的潜伏性单纯疱疹病毒（HSV）感染以及人类三叉神经节的潜伏性水痘-带状疱疹病毒（VZV）感染。在病毒潜伏期间（角膜接种HSV后四至五周）取出豚鼠的三叉神经节，在尸检时取出人类神经节。用放射性标记的HSV和VZV DNA探测神经节组织切片中的病毒特异性RNA。在22只潜伏感染豚鼠的46%的神经节以及10名人类受试者的33%的神经节中，仅在神经元上检测到杂交信号。这些结果支持了HSV和VZV潜伏期间发生了一些病毒转录的结论。
