Sánchez Jessica Andrea Albañil, Varela Antonio Sergio, Corcini Carine Dahl, da Silva Janaina Camacho, Primel Ednei Gilberto, Caldas Sergiane, Klein Roberta Daniele, Martins Camila De Martinez Gaspar
Programa de Pós-Graduação em Biologia de Ambientes Aquáticos Continentais, Instituto de Ciências Biológicas, Universidade Federal do Rio Grande, Av. Itália km 8, 96203-900, Rio Grande, RS, Brazil.
Programa de Pós-Graduação em Biologia de Ambientes Aquáticos Continentais, Instituto de Ciências Biológicas, Universidade Federal do Rio Grande, Av. Itália km 8, 96203-900, Rio Grande, RS, Brazil; Instituto de Ciências Biológicas, Universidade Federal do Rio Grande, Av. Itália km 8, 96203-900, Rio Grande, RS, Brazil.
Chemosphere. 2017 Jun;177:200-210. doi: 10.1016/j.chemosphere.2017.02.147. Epub 2017 Mar 1.
Roundup formulations are the most consumed glyphosate-based herbicides in the world. When applied, they may reach water bodies and exert toxicity toward non-target species. This study evaluated and compared the effects of different variations of Roundup on biochemical biomarkers as oxidative parameters and Acethylchorinesterase (AChE) activity, and sperm quality of the livebearing Jenynsia multidentata. Fish were acutely (96 h) exposed to Roundup Original (RO), Roundup Transorb (RT) and Roundup WG (RWG) at 0.0, 0.5, 1 and 5 mg L of nominal glyphosate. The highest mortality (60%) was observed for fish exposed to RT at the highest concentration tested and at 0.5 mg L non-mortality was observed, so this concentration was chosen for the experiments. Fish exposed to RO and RT (24 and 96 h) presented a state of oxidative imbalance, which caused lipid peroxidation (LPO) in their livers. Oxidative stress was more severe in RO treatment, which may be resulted in the highest hepathosomatic index at 96 h. However, fish exposed to RT presented a marked inhibition of AChE activity from membrane cells of muscle and brain tissues. Sperm quality was investigated in livebearing exposed (24 and 96 h) to the three formulations. Spermatozoa motility and concentration were affected by all formulations. Overall, Roundup formulations are harmful to the fish J. multidentata at 0.5 mg L of glyphosate; however, mechanisms and potential of toxicity are different between formulations. The J. multidentata also represents a sensitive species and a good regional bio-monitor.
农达制剂是全球使用最广泛的草甘膦基除草剂。施用后，它们可能进入水体并对非目标物种产生毒性。本研究评估并比较了不同变体的农达对生化生物标志物（作为氧化参数和乙酰胆碱酯酶（AChE）活性）以及卵胎生的多齿珍妮丽鱼精子质量的影响。将鱼急性（96小时）暴露于标称草甘膦浓度为0.0、0.5、1和5毫克/升的农达原药（RO）、农达转运体（RT）和农达WG（RWG）中。在测试的最高浓度下，暴露于RT的鱼死亡率最高（60%），而在0.5毫克/升时未观察到死亡，因此选择该浓度进行实验。暴露于RO和RT的鱼（24小时和96小时）呈现氧化失衡状态，导致其肝脏中的脂质过氧化（LPO）。RO处理中的氧化应激更为严重，这可能导致96小时时肝脏体指数最高。然而，暴露于RT的鱼肌肉和脑组织膜细胞中的AChE活性受到明显抑制。对暴露于三种制剂（24小时和96小时）的卵胎生鱼的精子质量进行了研究。所有制剂均影响精子活力和浓度。总体而言，在草甘膦浓度为0.5毫克/升时，农达制剂对多齿珍妮丽鱼有害；然而，不同制剂之间的毒性机制和潜力有所不同。多齿珍妮丽鱼也是一个敏感物种和良好的区域生物监测指标。
