Biotechnology of Animal and Human Reproduction (TechnoSperm), Institute of Food and Agricultural Technology, University of Girona, Girona, Spain.
Unit of Cell Biology, Department of Biology, Faculty of Sciences, University of Girona, C/Maria Aurèlia Campany, 69, Campus Montilivi, 17003, Girona, Spain.
Sci Rep. 2020 Jul 3;10(1):11026. doi: 10.1038/s41598-020-67538-w.
The wide use of glyphosate-based herbicides (GBHs) has become a matter of concern due to its potential harmful effects on human health, including men fertility. This study sought to investigate, using the pig as a model, the impact of pure glyphosate and its most known commercial formulation, Roundup, on sperm function and survival. With this purpose, fresh commercial semen doses were incubated with different concentrations (0-360 µg/mL) of glyphosate (GLY; exp. 1) or Roundup, at the equivalent GLY concentration (exp. 2), at 38 °C for 3 h. Glyphosate at 360 µg/mL significantly (P < 0.05) decreased sperm motility, viability, mitochondrial activity and acrosome integrity but had no detrimental effect at lower doses. On the other hand, Roundup did significantly (P < 0.05) reduce sperm motility at ≥ 5 µg/mL GLY-equivalent concentration; mitochondrial activity at ≥ 25 µg/mL GLY-equivalent concentration; and sperm viability and acrosome integrity at ≥ 100 µg/mL GLY-equivalent concentration as early as 1 h of incubation. In a similar fashion, GLY and Roundup did not inflict any detrimental effect on sperm DNA integrity. Taken together, these data indicate that, while both glyphosate and Roundup exert a negative impact on male gametes, Roundup is more toxic than its main component, glyphosate.
草甘膦基除草剂（GBHs）的广泛使用引起了人们的关注，因为它可能对人类健康造成潜在危害，包括男性生育能力。本研究旨在以猪为模型，研究纯草甘膦及其最知名的商业制剂农达（Roundup）对精子功能和存活的影响。为此，新鲜的商业精液剂量在 38°C 下用不同浓度（0-360µg/mL）的草甘膦（GLY；实验 1）或农达（Roundup，在等效 GLY 浓度下（实验 2）孵育 3 小时。360µg/mL 的草甘膦显著（P < 0.05）降低了精子的活力、活力、线粒体活性和顶体完整性，但在较低剂量下没有不良影响。另一方面，农达在≥5µg/mL GLY 等效浓度时显著（P < 0.05）降低了精子活力；在≥25µg/mL GLY 等效浓度时降低了线粒体活性；在≥100µg/mL GLY 等效浓度时降低了精子活力和顶体完整性早在孵育 1 小时就开始了。同样，草甘膦和农达对精子 DNA 完整性没有任何不良影响。总之，这些数据表明，尽管草甘膦和农达都对男性配子产生负面影响，但农达比其主要成分草甘膦更具毒性。
