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两种识别不对称核苷酸序列的II型甲基化酶基因的克隆:FokI和HgaI。

Cloning of two type II methylase genes that recognise asymmetric nucleotide sequences: FokI and HgaI.

作者信息

Nwankwo D, Wilson G

机构信息

New England Biolaboratories Inc, Beverly, MA 01915.

出版信息

Mol Gen Genet. 1987 Oct;209(3):570-4. doi: 10.1007/BF00331164.

DOI:10.1007/BF00331164
PMID:2828884
Abstract

The modification genes of Flavobacterium okeanokoites and Haemophilus galinarum have been cloned into the vector pBR322 and expressed in Escherichia coli cells. FokI methylase gene is contained on a 3.80 kb piece of F. okeanokoites DNA. Plasmid constructs carrying this fragment of DNA are resistant to digestion by FokI restriction endonuclease but are sensitive to cleavage by HindIII, EcoRI and PstI. Unmodified lambda DNA molecules, exposed in vitro to cell extracts prepared from cells habouring this plasmid, became resistant to digestion by FokI. The smallest HgaI methylase clone carries the pBR322 plasmid containing a 3.50 kb piece of H. galinarum DNA. This plasmid is resistant to digestion by HgaI. Neither the FokI nor the HgaI restriction endonuclease was detected in either clone. This is the first report of cloning modification genes whose protein products recognise asymmetric nucleotide sequences.

摘要

食海洋黄杆菌(Flavobacterium okeanokoites)和鸡嗜血杆菌(Haemophilus galinarum)的修饰基因已被克隆到载体pBR322中,并在大肠杆菌细胞中表达。食海洋黄杆菌的一段3.80 kb的DNA片段上含有FokI甲基化酶基因。携带该DNA片段的质粒构建体对FokI限制性内切酶的消化具有抗性,但对HindIII、EcoRI和PstI的切割敏感。在体外将未修饰的λDNA分子暴露于含有该质粒的细胞制备的细胞提取物中后,其对FokI的消化具有抗性。最小的HgaI甲基化酶克隆携带含有一段3.50 kb鸡嗜血杆菌DNA的pBR322质粒。该质粒对HgaI的消化具有抗性。在这两个克隆中均未检测到FokI或HgaI限制性内切酶。这是关于克隆其蛋白质产物识别不对称核苷酸序列的修饰基因的首次报道。

相似文献

1
Cloning of two type II methylase genes that recognise asymmetric nucleotide sequences: FokI and HgaI.两种识别不对称核苷酸序列的II型甲基化酶基因的克隆:FokI和HgaI。
Mol Gen Genet. 1987 Oct;209(3):570-4. doi: 10.1007/BF00331164.
2
Cloning and expression of the MspI restriction and modification genes.MspI 限制与修饰基因的克隆及表达
Gene. 1988 Apr 15;64(1):1-8. doi: 10.1016/0378-1119(88)90475-1.
3
Overproduction of the EcoRII endonuclease and methylase by Escherichia coli strains carrying recombinant plasmids constructed in vitro.携带体外构建的重组质粒的大肠杆菌菌株对EcoRII核酸内切酶和甲基化酶的过量生产。
Biochim Biophys Acta. 1981 Aug 27;655(1):102-6. doi: 10.1016/0005-2787(81)90072-1.
4
Cloning the modification methylase gene of Bacillus sphaericus R in Escherichia coli.在大肠杆菌中克隆球形芽孢杆菌R的修饰甲基化酶基因。
Gene. 1980 Aug;10(3):219-25. doi: 10.1016/0378-1119(80)90051-7.
5
The HgaI restriction-modification system contains two cytosine methylase genes responsible for modification of different DNA strands.HgaI 限制-修饰系统包含两个负责修饰不同 DNA 链的胞嘧啶甲基化酶基因。
J Biol Chem. 1991 Jul 25;266(21):13952-7.
6
Cloning of a restriction-modification system from Proteus vulgaris and its use in analyzing a methylase-sensitive phenotype in Escherichia coli.从普通变形杆菌中克隆一个限制修饰系统及其在分析大肠杆菌甲基化酶敏感性表型中的应用。
J Bacteriol. 1985 Nov;164(2):501-9. doi: 10.1128/jb.164.2.501-509.1985.
7
Nucleotide sequence of the FokI restriction-modification system: separate strand-specificity domains in the methyltransferase.FokI 限制-修饰系统的核苷酸序列:甲基转移酶中独立的链特异性结构域
Gene. 1989 Aug 15;80(2):193-208. doi: 10.1016/0378-1119(89)90284-9.
8
The fokI restriction-modification system. I. Organization and nucleotide sequences of the restriction and modification genes.福克I型限制修饰系统。I. 限制与修饰基因的组织及核苷酸序列
J Biol Chem. 1989 Apr 5;264(10):5751-6.
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Cloning of the modification methylase gene of Bacillus centrosporus in Escherichia coli.嗜中芽孢杆菌修饰甲基化酶基因在大肠杆菌中的克隆
Gene. 1982 Dec;20(2):197-204. doi: 10.1016/0378-1119(82)90038-5.
10
Cloning the DdeI restriction-modification system using a two-step method.采用两步法克隆DdeI限制修饰系统。
Nucleic Acids Res. 1986 Oct 24;14(20):7939-51. doi: 10.1093/nar/14.20.7939.

引用本文的文献

1
Alteration of the cleavage distance of Fok I restriction endonuclease by insertion mutagenesis.通过插入诱变改变福克I型限制性内切酶的切割距离。
Proc Natl Acad Sci U S A. 1993 Apr 1;90(7):2764-8. doi: 10.1073/pnas.90.7.2764.
2
Effect of site-specific methylation on restriction endonucleases and DNA modification methyltransferases.位点特异性甲基化对限制性内切核酸酶和DNA修饰甲基转移酶的影响。
Nucleic Acids Res. 1993 Jul 1;21(13):3139-54. doi: 10.1093/nar/21.13.3139.
3
Effect of site-specific modification on restriction endonucleases and DNA modification methyltransferases.

本文引用的文献

1
Characterization of the genes coding for the Eco RV restriction and modification system of Escherichia coli.大肠杆菌Eco RV限制与修饰系统编码基因的特性分析。
Nucleic Acids Res. 1984 Apr 25;12(8):3659-76. doi: 10.1093/nar/12.8.3659.
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Restriction and modification enzymes and their recognition sequences.限制酶和修饰酶及其识别序列。
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Cloned restriction/modification system from Pseudomonas aeruginosa.来自铜绿假单胞菌的克隆限制/修饰系统。
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Effect of site-specific methylation on DNA modification methyltransferases and restriction endonucleases.位点特异性甲基化对DNA修饰甲基转移酶和限制性内切核酸酶的影响。
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Characterization and expression of the Escherichia coli Mrr restriction system.大肠杆菌Mrr限制系统的特性与表达
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7
Site-specific methylation: effect on DNA modification methyltransferases and restriction endonucleases.位点特异性甲基化:对DNA修饰甲基转移酶和限制性内切酶的影响。
Nucleic Acids Res. 1991 Apr 25;19 Suppl(Suppl):2045-71. doi: 10.1093/nar/19.suppl.2045.
8
Functional domains in Fok I restriction endonuclease.福克I型限制性内切核酸酶中的功能结构域。
Proc Natl Acad Sci U S A. 1992 May 15;89(10):4275-9. doi: 10.1073/pnas.89.10.4275.
9
Effect of site-specific methylation on DNA modification methyltransferases and restriction endonucleases.位点特异性甲基化对DNA修饰甲基转移酶和限制性内切酶的影响。
Nucleic Acids Res. 1992 May 11;20 Suppl(Suppl):2145-57. doi: 10.1093/nar/20.suppl.2145.
Proc Natl Acad Sci U S A. 1983 Jan;80(2):402-6. doi: 10.1073/pnas.80.2.402.
4
Cloning and expression of the Pst I restriction-modification system in Escherichia coli.Pst I 限制修饰系统在大肠杆菌中的克隆与表达。
Proc Natl Acad Sci U S A. 1981 Mar;78(3):1503-7. doi: 10.1073/pnas.78.3.1503.
5
Cloning of the MspI modification enzyme. The site of modification and its effects on cleavage by MspI and HpaII.MspI 甲基化酶的克隆。甲基化位点及其对 MspI 和 HpaII 切割的影响。
J Biol Chem. 1983 Jan 25;258(2):1235-41.
6
Alteration of apparent restriction endonuclease recognition specificities by DNA methylases.DNA甲基化酶对表观限制性内切酶识别特异性的改变。
Nucleic Acids Res. 1984 Jul 11;12(13):5165-73. doi: 10.1093/nar/12.13.5165.
7
Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an opern circular DNA form.大肠杆菌中的超螺旋环状DNA-蛋白质复合物:纯化及诱导转化为开放环状DNA形式
Proc Natl Acad Sci U S A. 1969 Apr;62(4):1159-66. doi: 10.1073/pnas.62.4.1159.
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Nonchromosomal antibiotic resistance in bacteria: genetic transformation of Escherichia coli by R-factor DNA.细菌中的非染色体抗生素抗性:R 因子 DNA 对大肠杆菌的遗传转化
Proc Natl Acad Sci U S A. 1972 Aug;69(8):2110-4. doi: 10.1073/pnas.69.8.2110.
9
Cloning, sequencing, in vivo promoter mapping, and expression in Escherichia coli of the gene for the HhaI methyltransferase.HhaI甲基转移酶基因的克隆、测序、体内启动子定位及在大肠杆菌中的表达。
J Biol Chem. 1987 Apr 5;262(10):4770-7.
10
Escherichia coli K-12 restricts DNA containing 5-methylcytosine.大肠杆菌K-12会限制含有5-甲基胞嘧啶的DNA。
Proc Natl Acad Sci U S A. 1986 Dec;83(23):9070-4. doi: 10.1073/pnas.83.23.9070.